Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 2% Sucrose
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab87011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
RelevanceHSD17B14 belongs to the short-chain dehydrogenases/reductases (SDR) family. It has NAD-dependent 17-beta-hydroxysteroid dehydrogenase activity and converts oestradiol to oestrone. The physiological substrate is not known. HSD17B14 acts on oestradiol and 5-androstene-3-beta,17-beta-diol (in vitro). It is highly expressed in brain, placenta, liver and kidney.
- 17 beta HSD 14 antibody
- 17 beta hydroxysteroid dehydrogenase 14 antibody
- 17 beta hydroxysteroid dehydrogenase DHRS10 antibody
Anti-HSD17B14 antibody (ab87011) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Gel concentration 12%
Immunohistochemical analysis of Human bladder tissue, staining HSD17B14 with ab87011.
Tissue was fixed with formaldehyde and blocked with 1% BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/25 in TEG) for 1 hour at 22 °C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
ab87011 has not yet been referenced specifically in any publications.