Anti-HSD17B2 antibody (ab196784)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-HSD17B2 antibody
See all HSD17B2 primary antibodies -
Description
Rabbit polyclonal to HSD17B2 -
Host species
Rabbit -
Specificity
ab196784 detects endogenous level of total HSD17B2 protein. -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human HSD17B2 (C terminal).
Database link: P37059 -
Positive control
- SW480 cell extracts. Mouse liver, kidney, craniofacial and intestine extracts. U2OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab196784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/200. | |
| IHC-P | 1/50 - 1/200. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 43 kDa. |
Target
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Function
Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3294 Human
- Entrez Gene: 15486 Mouse
- Omim: 109685 Human
- SwissProt: P37059 Human
- SwissProt: P51658 Mouse
- Unigene: 162795 Human
- Unigene: 276466 Mouse
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Alternative names
- 17 beta HSD 2 antibody
- 17-beta-HSD 2 antibody
- 17-beta-hydroxysteroid dehydrogenase type 2 antibody
see all
Images
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Western blot - Anti-HSD17B2 antibody (ab196784)All lanes : Anti-HSD17B2 antibody (ab196784) at 1/500 dilution
Lane 1 : SW480 cell extracts
Lane 2 : Mouse liver cell extracts
Lane 3 : Mouse kidney cell extracts
Lane 4 : Mouse craniofacial cell extracts
Lane 5 : Mouse intestines cell extracts
Predicted band size: 43 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-HSD17B2 antibody (ab196784)Immunofluorescent analysis of U20S cells, labeling HSD17B2 (green) with ab196784 at 1/50 dilution. Blue: DAPI for nuclear staining.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhang K et al. CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice. J Clin Invest 129:2333-2350 (2019). Read more (PubMed: 31063987) »
