Anti-HSD17B2 antibody (ab196784)
Key features and details
- Rabbit polyclonal to HSD17B2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HSD17B2 antibody
See all HSD17B2 primary antibodies -
Description
Rabbit polyclonal to HSD17B2 -
Host species
Rabbit -
Specificity
ab196784 detects endogenous level of total HSD17B2 protein. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human HSD17B2 (C terminal).
Database link: P37059 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab196784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | 1/50 - 1/200. | |
WB | 1/500 - 1/2000. Predicted molecular weight: 43 kDa. |
Target
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Function
Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3294 Human
- Entrez Gene: 15486 Mouse
- Omim: 109685 Human
- SwissProt: P37059 Human
- SwissProt: P51658 Mouse
- Unigene: 162795 Human
- Unigene: 276466 Mouse
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Alternative names
- 17 beta HSD 2 antibody
- 17-beta-HSD 2 antibody
- 17-beta-hydroxysteroid dehydrogenase type 2 antibody
see all
Images
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All lanes : Anti-HSD17B2 antibody (ab196784) at 1/500 dilution
Lane 1 : SW480 cell extracts
Lane 2 : Mouse liver cell extracts
Lane 3 : Mouse kidney cell extracts
Lane 4 : Mouse craniofacial cell extracts
Lane 5 : Mouse intestines cell extracts
Predicted band size: 43 kDa -
Immunofluorescent analysis of U20S cells, labeling HSD17B2 (green) with ab196784 at 1/50 dilution. Blue: DAPI for nuclear staining.
Protocols
Datasheets and documents
References (1)
ab196784 has been referenced in 1 publication.
- Zhang K et al. CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice. J Clin Invest 129:2333-2350 (2019). PubMed: 31063987