Key features and details
- Rabbit polyclonal to HSD17B2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HSD17B2 antibody
See all HSD17B2 primary antibodies
DescriptionRabbit polyclonal to HSD17B2
Specificityab196784 detects endogenous level of total HSD17B2 protein.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human HSD17B2 (C terminal).
Database link: P37059
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 43 kDa.|
FunctionCapable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
- Information by UniProt
- 17 beta HSD 2 antibody
- 17-beta-HSD 2 antibody
- 17-beta-hydroxysteroid dehydrogenase type 2 antibody
All lanes : Anti-HSD17B2 antibody (ab196784) at 1/500 dilution
Lane 1 : SW480 cell extracts
Lane 2 : Mouse liver cell extracts
Lane 3 : Mouse kidney cell extracts
Lane 4 : Mouse craniofacial cell extracts
Lane 5 : Mouse intestines cell extracts
Predicted band size: 43 kDa
Immunofluorescent analysis of U20S cells, labeling HSD17B2 (green) with ab196784 at 1/50 dilution. Blue: DAPI for nuclear staining.
ab196784 has been referenced in 1 publication.
- Zhang K et al. CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice. J Clin Invest 129:2333-2350 (2019). PubMed: 31063987