Anti-HSD17B2 antibody (ab196784)

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Western blot - Anti-HSD17B2 antibody (ab196784)
  • Immunocytochemistry/ Immunofluorescence - Anti-HSD17B2 antibody (ab196784)

Key features and details

  • Rabbit polyclonal to HSD17B2
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-HSD17B2 antibody
    See all HSD17B2 primary antibodies

  • Description

    Rabbit polyclonal to HSD17B2

  • Host species

    Rabbit

  • Specificity

    ab196784 detects endogenous level of total HSD17B2 protein.

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment corresponding to Human HSD17B2 (C terminal).
    Database link: P37059

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    PBS without Mg2+ and Ca2+.
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 43 kDa.

Target

  • Function

    Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.

  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 17 beta HSD 2 antibody
      • 17-beta-HSD 2 antibody
      • 17-beta-hydroxysteroid dehydrogenase type 2 antibody
      • 20 alpha HSD antibody
      • 20 alpha hydroxysteroid dehydrogenase antibody
      • 20 alpha-hydroxysteroid dehydrogenase antibody
      • 20-alpha-HSD antibody
      • DHB2_HUMAN antibody
      • E2DH antibody
      • EDH17B2 antibody
      • Estradiol 17-beta-dehydrogenase 2 antibody
      • HSD17 antibody
      • Hsd17b2 antibody
      • Hydroxysteroid (17 beta) dehydrogenase 2 antibody
      • Microsomal 17 beta hydroxysteroid dehydrogenase antibody
      • Microsomal 17-beta-hydroxysteroid dehydrogenase antibody
      • SDR9C2 antibody
      • Short chain dehydrogenase/reductase family 9C, member 2 antibody
      • Testosterone 17 beta dehydrogenase antibody
      • Testosterone 17-beta-dehydrogenase antibody
      see all

    Images

    • Western blot - Anti-HSD17B2 antibody (ab196784)
      Western blot - Anti-HSD17B2 antibody (ab196784)
      All lanes : Anti-HSD17B2 antibody (ab196784) at 1/500 dilution

      Lane 1 : SW480 cell extracts
      Lane 2 : Mouse liver cell extracts
      Lane 3 : Mouse kidney cell extracts
      Lane 4 : Mouse craniofacial cell extracts
      Lane 5 : Mouse intestines cell extracts

      Predicted band size: 43 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-HSD17B2 antibody (ab196784)
      Immunocytochemistry/ Immunofluorescence - Anti-HSD17B2 antibody (ab196784)

      Immunofluorescent analysis of U20S cells, labeling HSD17B2 (green) with ab196784 at 1/50 dilution. Blue: DAPI for nuclear staining. 

    References (1)

    Publishing research using ab196784? Please let us know so that we can cite the reference in this datasheet.

    ab196784 has been referenced in 1 publication.

    • Zhang K  et al. CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice. J Clin Invest 129:2333-2350 (2019). PubMed: 31063987

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