Key features and details
- Mouse monoclonal [2D3BB5BF10] to HSD17B4
- Suitable for: WB, ICC/IF, In-Cell ELISA, IP
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-HSD17B4 antibody [2D3BB5BF10]
See all HSD17B4 primary antibodies
DescriptionMouse monoclonal [2D3BB5BF10] to HSD17B4
Tested applicationsSuitable for: WB, ICC/IF, In-Cell ELISA, IPmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Tissue, cells or virus. This information is considered to be commercially sensitive.
- Human liver and heart homogenates; Hela, HepG2, HDFn, HEK293T, Jurkat and SH-SY5Y cell lysate; HDFn cells
This antibody clone is manufactured by Abcam.
This monoclonal antibody to HSD17B4 has been knockout validated in Western blot. The expected band was observed in wild type cells and the band was not seen in knockout cells.
This product was previously marketed under the MitoSciences sub-brand.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesPurity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab128565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 79 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|In-Cell ELISA||Use a concentration of 1 - 5 µg/ml.|
|IP||Use at an assay dependent concentration.|
FunctionBifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.
Tissue specificityPresent in many tissues with highest concentrations in liver, heart, prostate and testis.
PathwayLipid metabolism; fatty acid beta-oxidation.
Involvement in diseaseD-bifunctional protein deficiency
Perrault syndrome 1
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 MaoC-like domain.
Contains 1 SCP2 domain.
- Information by UniProt
- 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody
- 17 beta HSD 4 antibody
- 17 beta HSD IV antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: HSD17B4 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab128565 observed at 85 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab128565 was shown to specifically react with HSD17B4 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when HSD17B4 knockout cells were examined. Wild-type and HSD17B4 knockout samples were subjected to SDS-PAGE. Ab128565 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunofluorescent staining of HSD17B4 in HDFn cells (human) using ab126565.
The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab128565, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the peroxisome.
Immunoprecipitation using ab128565.
The protein is found both as a full length peptide and in a cleaved version.
Lane 1: Ladder
Lane 2: Human Liver Homogenate.
All lanes : Anti-HSD17B4 antibody [2D3BB5BF10] (ab128565) at 1 µg/ml
Lane 1 : ladder
Lane 2 : Human Liver Homoginate at 10 µg
Lane 3 : Human Heart Homogenate at 10 µg
Lane 4 : Hela cell lysate at 10 µg
Lane 5 : HepG2 cell lysate at 10 µg
Lane 6 : HDFn cell lysate at 10 µg
Lane 7 : Hek293T cell lysate at 10 µg
Lane 8 : Jurket cell lysate at 10 µg
Lane 9 : SH-SY5Y cell lysate at 10 µg
Predicted band size: 79 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab128565 has been referenced in 2 publications.
- Violante S et al. Peroxisomes can oxidize medium- and long-chain fatty acids through a pathway involving ABCD3 and HSD17B4. FASEB J 33:4355-4364 (2019). PubMed: 30540494
- Zhang Y et al. SIRT1 activation alleviates brain microvascular endothelial dysfunction in peroxisomal disorders. Int J Mol Med 44:995-1005 (2019). PubMed: 31257461