Overview

  • Product name

    Anti-HSD17B4 antibody [2D3BB5BF10]
    See all HSD17B4 primary antibodies
  • Description

    Mouse monoclonal [2D3BB5BF10] to HSD17B4
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, In-Cell ELISA, IPmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Human liver and heart homogenates; Hela, HepG2, HDFn, HEK293T, Jurkat and SH-SY5Y cell lysate; HDFn cells
  • General notes

    This antibody clone is manufactured by Abcam.

    This monoclonal antibody to HSD17B4 has been knockout validated in Western blot. The expected band was observed in wild type cells and the band was not seen in knockout cells.

    This product was previously marketed under the MitoSciences sub-brand.

     

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab128565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 79 kDa.
ICC/IF Use a concentration of 5 µg/ml.
In-Cell ELISA Use a concentration of 1 - 5 µg/ml.
IP Use at an assay dependent concentration.

Target

  • Function

    Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.
  • Tissue specificity

    Present in many tissues with highest concentrations in liver, heart, prostate and testis.
  • Pathway

    Lipid metabolism; fatty acid beta-oxidation.
  • Involvement in disease

    D-bifunctional protein deficiency
    Perrault syndrome 1
  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    Contains 1 MaoC-like domain.
    Contains 1 SCP2 domain.
  • Cellular localization

    Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody
    • 17 beta HSD 4 antibody
    • 17 beta HSD IV antibody
    • 17 beta hydroxysteroid dehydrogenase 4 antibody
    • 17-beta-HSD 4 antibody
    • 17-beta-hydroxysteroid dehydrogenase 4 antibody
    • 17beta estradiol dehydrogenase type IV antibody
    • 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholest 24 enoyl CoA hydratase antibody
    • 3-alpha antibody
    • 7-alpha antibody
    • Beta hydroxyacyl dehydrogenase antibody
    • Beta keto reductase antibody
    • D 3 hydroxyacyl CoA dehydratase antibody
    • D bifunctional protein antibody
    • D bifunctional protein peroxisomal antibody
    • D-3-hydroxyacyl CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein antibody
    • D-bifunctional protein antibody
    • D-bifunctional protein, peroxisomal antibody
    • DBP antibody
    • DBP, peroxisomal antibody
    • DHB4_HUMAN antibody
    • EDH17B4 antibody
    • Enoyl-CoA hydratase 2 antibody
    • Hsd17b4 antibody
    • Hydroxysteroid (17-beta) Dehydrogenase 4 antibody
    • MFE 2 antibody
    • MFE-2 antibody
    • MPF-2 antibody
    • Multifunctional protein 2 antibody
    • Peroxisomal multifunctional enzyme type 2 antibody
    • Peroxisomal multifunctional protein 2 antibody
    • PRLTS1 antibody
    • SDR8C1 antibody
    • Short chain dehydrogenase/reductase family 8C member 1 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: HSD17B4 knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: A431 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab128565 observed at 85 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab128565 was shown to specifically react with HSD17B4 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when HSD17B4 knockout cells were examined. Wild-type and HSD17B4 knockout samples were subjected to SDS-PAGE.  Ab128565 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunofluorescent staining of HSD17B4 in HDFn cells (human) using ab126565.
    The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab128565, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the peroxisome.

  • Immunoprecipitation using ab128565.

    The protein is found both as a full length peptide and in a cleaved version.

    Lane 1: Ladder
    Lane 2: Human Liver Homogenate.
  • All lanes : Anti-HSD17B4 antibody [2D3BB5BF10] (ab128565) at 1 µg/ml

    Lane 1 : ladder
    Lane 2 : Human Liver Homoginate at 10 µg
    Lane 3 : Human Heart Homogenate at 10 µg
    Lane 4 : Hela cell lysate at 10 µg
    Lane 5 : HepG2 cell lysate at 10 µg
    Lane 6 : HDFn cell lysate at 10 µg
    Lane 7 : Hek293T cell lysate at 10 µg
    Lane 8 : Jurket cell lysate at 10 µg
    Lane 9 : SH-SY5Y cell lysate at 10 µg

    Predicted band size: 79 kDa

References

This product has been referenced in:

  • Violante S  et al. Peroxisomes can oxidize medium- and long-chain fatty acids through a pathway involving ABCD3 and HSD17B4. FASEB J 33:4355-4364 (2019). Read more (PubMed: 30540494) »
See 1 Publication for this product

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