Overview

  • Product name

    Anti-HSD17B4 antibody [2D3BB5BF10] (Alexa Fluor® 647)
    See all HSD17B4 primary antibodies
  • Description

    Mouse monoclonal [2D3BB5BF10] to HSD17B4 (Alexa Fluor® 647)
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Tissue, cells or virus corresponding to Human HSD17B4.

  • Positive control

    • ICC/IF: HeLa and HepG2 cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab198320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HeLa and HepG2 cells fixed with 4% formaldehyde (10 min).

Target

  • Function

    Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.
  • Tissue specificity

    Present in many tissues with highest concentrations in liver, heart, prostate and testis.
  • Pathway

    Lipid metabolism; fatty acid beta-oxidation.
  • Involvement in disease

    D-bifunctional protein deficiency
    Perrault syndrome 1
  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    Contains 1 MaoC-like domain.
    Contains 1 SCP2 domain.
  • Cellular localization

    Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody
    • 17 beta HSD 4 antibody
    • 17 beta HSD IV antibody
    • 17 beta hydroxysteroid dehydrogenase 4 antibody
    • 17-beta-HSD 4 antibody
    • 17-beta-hydroxysteroid dehydrogenase 4 antibody
    • 17beta estradiol dehydrogenase type IV antibody
    • 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholest 24 enoyl CoA hydratase antibody
    • 3-alpha antibody
    • 7-alpha antibody
    • Beta hydroxyacyl dehydrogenase antibody
    • Beta keto reductase antibody
    • D 3 hydroxyacyl CoA dehydratase antibody
    • D bifunctional protein antibody
    • D bifunctional protein peroxisomal antibody
    • D-3-hydroxyacyl CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein antibody
    • D-bifunctional protein antibody
    • D-bifunctional protein, peroxisomal antibody
    • DBP antibody
    • DBP, peroxisomal antibody
    • DHB4_HUMAN antibody
    • EDH17B4 antibody
    • Enoyl-CoA hydratase 2 antibody
    • Hsd17b4 antibody
    • Hydroxysteroid (17-beta) Dehydrogenase 4 antibody
    • MFE 2 antibody
    • MFE-2 antibody
    • MPF-2 antibody
    • Multifunctional protein 2 antibody
    • Peroxisomal multifunctional enzyme type 2 antibody
    • Peroxisomal multifunctional protein 2 antibody
    • PRLTS1 antibody
    • SDR8C1 antibody
    • Short chain dehydrogenase/reductase family 8C member 1 antibody
    see all

Images

  • ab198320 staining HSD17B4 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198320 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • ab198320 staining HSD17B4 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198320 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab198320 has not yet been referenced specifically in any publications.

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