Overview

  • Product name

  • Description

    Rabbit polyclonal to HSD17B4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human HSD17B4 aa 600-650. The exact sequence is proprietary.
    Database link: P51659

  • Positive control

    • IP: HEK-293T whole cell lysate.
  • General notes

    ab226220 has not performed satisfactorily when used for WB of HSD17B4 in crude preparations (e.g. whole cell lysate).  This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of HSD17B4.

Properties

Applications

Our Abpromise guarantee covers the use of ab226220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.
    • Tissue specificity

      Present in many tissues with highest concentrations in liver, heart, prostate and testis.
    • Pathway

      Lipid metabolism; fatty acid beta-oxidation.
    • Involvement in disease

      D-bifunctional protein deficiency
      Perrault syndrome 1
    • Sequence similarities

      Belongs to the short-chain dehydrogenases/reductases (SDR) family.
      Contains 1 MaoC-like domain.
      Contains 1 SCP2 domain.
    • Cellular localization

      Peroxisome.
    • Information by UniProt
    • Database links

    • Alternative names

      • 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody
      • 17 beta HSD 4 antibody
      • 17 beta HSD IV antibody
      • 17 beta hydroxysteroid dehydrogenase 4 antibody
      • 17-beta-HSD 4 antibody
      • 17-beta-hydroxysteroid dehydrogenase 4 antibody
      • 17beta estradiol dehydrogenase type IV antibody
      • 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholest 24 enoyl CoA hydratase antibody
      • 3-alpha antibody
      • 7-alpha antibody
      • Beta hydroxyacyl dehydrogenase antibody
      • Beta keto reductase antibody
      • D 3 hydroxyacyl CoA dehydratase antibody
      • D bifunctional protein antibody
      • D bifunctional protein peroxisomal antibody
      • D-3-hydroxyacyl CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein antibody
      • D-bifunctional protein antibody
      • D-bifunctional protein, peroxisomal antibody
      • DBP antibody
      • DBP, peroxisomal antibody
      • DHB4_HUMAN antibody
      • EDH17B4 antibody
      • Enoyl-CoA hydratase 2 antibody
      • Hsd17b4 antibody
      • Hydroxysteroid (17-beta) Dehydrogenase 4 antibody
      • MFE 2 antibody
      • MFE-2 antibody
      • MPF-2 antibody
      • Multifunctional protein 2 antibody
      • Peroxisomal multifunctional enzyme type 2 antibody
      • Peroxisomal multifunctional protein 2 antibody
      • PRLTS1 antibody
      • SDR8C1 antibody
      • Short chain dehydrogenase/reductase family 8C member 1 antibody
      see all

    Images

    • HSD17B4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226220 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226220 at 0.4 µg/ml.

      Lane 1: ab226220 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 30 seconds.

    References

    ab226220 has not yet been referenced specifically in any publications.

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