Product nameAnti-HSD17B4 antibody
See all HSD17B4 primary antibodies
DescriptionRabbit polyclonal to HSD17B4
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey, Rhesus monkey, Orangutan
Synthetic peptide within Human HSD17B4 aa 600-650. The exact sequence is proprietary.
Database link: P51659
- IP: HEK-293T whole cell lysate.
ab226220 has not performed satisfactorily when used for WB of HSD17B4 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of HSD17B4.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226220 was affinity purified using an epitope specific to HSD17B4 immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab226220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionBifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.
Tissue specificityPresent in many tissues with highest concentrations in liver, heart, prostate and testis.
PathwayLipid metabolism; fatty acid beta-oxidation.
Involvement in diseaseD-bifunctional protein deficiency
Perrault syndrome 1
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 MaoC-like domain.
Contains 1 SCP2 domain.
- Information by UniProt
- 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody
- 17 beta HSD 4 antibody
- 17 beta HSD IV antibody
HSD17B4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226220 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226220 at 0.4 µg/ml.
Lane 1: ab226220 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab226220 has not yet been referenced specifically in any publications.