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I purchased the products ab82382 Rabbit polyclonal to Sterol carrier protein 2, and ab97971 Rabbit polyclonal to hydroxysteroid (17-beta) dehydrogenase 4.
I am going to try a Western Blot next using the antibodies but I have not been able to find on your references the appropriate buffer for dilution, in case of the storage buffer it is marked upon request.
Could you please specify me which one to use for each case?
Asked on Apr 06 2012
Thank you for yourenquiry.
We would generallyrecommend using 1-3% BSA as the dilutioin buffer, and 5%BSA as the blocking buffer. Please see our WB protocol below for more details on buffer preparation and etc.
I hope this information will be helpful. If there is anything else that I can help you with please do not hesitate to contact me.
Have a nice day!
Answered on Apr 06 2012