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Thank you very much again for your interest and kind answer.
I actually wanted to know your opinion about the quality of the results. For SCP-2 looksdefinitelygood enough with blocking 1. Still, MFE-2 signal is not as strong as in your data (using both HeLa)
I am going to try a different concentration with the antibodyand in case the signal is still faint I will mail you with theinquiry.
Asked on May 24 2012
Thank you for getting back to me.
I hope your next experiment will produce desirable results. Also, in order to increase the band intensity, you may want to consider increase the amount of protein, and also try using the same blocking buffer for primary and secondary antibody dilution.
Answered on May 24 2012