Overview

  • Product name

  • Description

    Mouse polyclonal to HSD17B6
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    HSD17B6 full length human protein (NP_003716.2)

  • Positive control

    • Human liver tissue lysate and HSD17B6 transfected 293T cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.

Target

  • Function

    NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha-androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids.
  • Tissue specificity

    Detected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain.
  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localization

    Microsome membrane. Early endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 17-beta-HSD 6 antibody
    • 17-beta-HSD6 antibody
    • 17-beta-hydroxysteroid dehydrogenase type 6 antibody
    • 3 hydroxysteroid epimerase antibody
    • 3(alpha >beta) hydroxysteroid epimerase antibody
    • 3(alpha >beta) hydroxysteroid epimerasel antibody
    • 3-alpha->beta-HSE antibody
    • 3-alpha->beta-hydroxysteroid epimerase antibody
    • H17B6_HUMAN antibody
    • HSD17B6 antibody
    • HSE antibody
    • Hydroxysteroid (17 beta) dehydrogenase 6 antibody
    • Hydroxysteroid (17 beta) dehydrogenase 6 homolog (mouse) antibody
    • Hydroxysteroid 17 beta dehydrogenase 6 antibody
    • NAD+ dependent 3 alpha hydroxysteroid dehydrogenase 3 hydroxysteroid epimerase antibody
    • NAD+ dependent 3 alpha hydroxysteroid dehydrogenase antibody
    • Oxidative 3 alpha hydroxysteroid dehydrogenase antibody
    • Oxidative 3-alpha hydroxysteroid dehydrogenase antibody
    • Oxidoreductase antibody
    • Retinol dehydrogenase antibody
    • RODH antibody
    • SDR9C6 antibody
    • Short chain dehydrogenase/reductase family 9C, member 6 antibody
    see all

Images

  • Anti-HSD17B6 antibody (ab88892) at 1/500 dilution + Human liver tissue lysate at 50 µg

    Predicted band size: 36 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-HSD17B6 antibody (ab88892) at 1/500 dilution

    Lane 1 : HSD17B6 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 36 kDa
    Observed band size: 32 kDa why is the actual band size different from the predicted?

References

ab88892 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

We are expecting the return of the 2 vials of ab62221 to our Cambridge, MA office. As requested, I have issued a free of charge replacement of 2 vials of ab88892. These are backordered, but you can expect them on Feb 20th. To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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