Recombinant
RabMAb

Recombinant Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)

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Overview

  • Product name

    Anti-HSD17B8 antibody [EPR12084(B)]
    See all HSD17B8 primary antibodies

  • Description

    Rabbit monoclonal [EPR12084(B)] to HSD17B8

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to amino acid residues in Human HSD17B8 (Q92506).

  • Positive control

    • Human fetal liver, HepG2, BxPC-3 and JAR cell lysates. JAR cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone. The heteroteramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria.

    • Tissue specificity

      Highly expressed in placenta, liver and pancreas, lower in the skeletal muscle and kidney. Widely expressed.

    • Pathway

      Steroid biosynthesis; estrogen biosynthesis.
      Lipid metabolism; fatty acid biosynthesis.

    • Sequence similarities

      Belongs to the short-chain dehydrogenases/reductases (SDR) family.

    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt

    • Database links

      • Alternative names

        • 17 beta HSD 8 antibody
        • 17 beta hydroxysteroid dehydrogenase 8 antibody
        • 17-beta-HSD 8 antibody
        • 17-beta-hydroxysteroid dehydrogenase 8 antibody
        • 17-beta-hydroxysteroid dehydrogenase VIII antibody
        • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
        • 3-oxoacyl-acyl-carrier protein reductase, E. coli, homolog of antibody
        • Beta ketoacyl [acyl carrier protein] reductase like antibody
        • D6S2245E antibody
        • DHB8_HUMAN antibody
        • dJ1033B10.9 antibody
        • Estradiol 17 beta dehydrogenase 8 antibody
        • Estradiol 17-beta-dehydrogenase 8 antibody
        • Estrogen 17 oxidoreductase antibody
        • FABG antibody
        • FabG, E. coli, homolog of antibody
        • FabG-like antibody
        • FABGL antibody
        • H2 KE6 antibody
        • H2-KE6 antibody
        • HKE6 antibody
        • HSD17B8 antibody
        • Hydroxysteroid (17 beta) dehydrogenase 8 antibody
        • Ke-6 antibody
        • KE6 antibody
        • KE6, mouse homolog of antibody
        • Protein Ke6 antibody
        • Really interesting new gene 2 protein antibody
        • RING2 antibody
        • SDR30C1 antibody
        • Short chain dehydrogenase/reductase family 30C member 1 antibody
        • Testosterone 17 beta dehydrogenase 8 antibody
        • Testosterone 17-beta-dehydrogenase 8 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
        Immunocytochemistry/ Immunofluorescence - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
        Immunofluorescent analysis of JAR cells labelling HSD17B8 with ab169752 at 1/100 dilution.
      • Western blot - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
        Western blot - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
        All lanes : Anti-HSD17B8 antibody [EPR12084(B)] (ab169752) at 1/10000 dilution

        Lane 1 : Fetal liver lysate
        Lane 2 : HepG2 lysate
        Lane 3 : Bx-PC-3 lysate
        Lane 4 : JAR lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 26 kDa

      • Immunoprecipitation - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
        Immunoprecipitation - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
        Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate labeling HSD17B8 with ab169752 at 1/10 dilution or (2) 1X PBS (negative control. HRP-conjugated anti-rabbit IgG preferentially detected the non-reduced form of Rabbit IgG.

      References

      ab169752 has not yet been referenced specifically in any publications.

      Publishing research using ab169752? Please let us know so that we can cite the reference in this datasheet.

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