Recombinant Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
![Immunocytochemistry/ Immunofluorescence - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)](https://www.abcam.com/ps/products/169/ab169752/Images/HSD17B8-Primary-antibodies-ab169752-1.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12084(B)] to HSD17B8
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HSD17B8 antibody [EPR12084(B)]
See all HSD17B8 primary antibodies -
Description
Rabbit monoclonal [EPR12084(B)] to HSD17B8 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, HepG2, BxPC-3 and JAR cell lysates. JAR cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12084(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
|
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| WB |
1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
|
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| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/250. |
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WB
1/10000 - 1/50000. Predicted molecular weight: 26 kDa. |
|
IP
1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt or IHC-P.
Target
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Function
NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone. The heteroteramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria. -
Tissue specificity
Highly expressed in placenta, liver and pancreas, lower in the skeletal muscle and kidney. Widely expressed. -
Pathway
Steroid biosynthesis; estrogen biosynthesis.
Lipid metabolism; fatty acid biosynthesis. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 7923 Human
- Omim: 601417 Human
- SwissProt: Q92506 Human
- Unigene: 415058 Human
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Alternative names
- 17 beta HSD 8 antibody
- 17 beta hydroxysteroid dehydrogenase 8 antibody
- 17-beta-HSD 8 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)Immunofluorescent analysis of JAR cells labelling HSD17B8 with ab169752 at 1/100 dilution.
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![Western blot - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)](https://www.abcam.com/ps/products/169/ab169752/Images/HSD17B8-Primary-antibodies-ab169752-2.jpg)
Western blot - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)All lanes : Anti-HSD17B8 antibody [EPR12084(B)] (ab169752) at 1/10000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : Bx-PC-3 lysate
Lane 4 : JAR lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Immunoprecipitation - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)](https://www.abcam.com/ps/products/169/ab169752/Images/HSD17B8-Primary-antibodies-ab169752-3.jpg)
Immunoprecipitation - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate labeling HSD17B8 with ab169752 at 1/10 dilution or (2) 1X PBS (negative control. HRP-conjugated anti-rabbit IgG preferentially detected the non-reduced form of Rabbit IgG. -
![Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)](https://www.abcam.com/ps/products/169/ab169752/Images/ab169752-4-benefits-of-recombinant-antibodies.png)
Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab169752 has not yet been referenced specifically in any publications.
