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    hsd17b8-antibody-epr12084b-ab169752.pdf

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RecombinantRabMAb

Recombinant Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)

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Immunocytochemistry/ Immunofluorescence - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
  • Western blot - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
  • Immunoprecipitation - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
  • Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12084(B)] to HSD17B8
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-HSD17B8 antibody [EPR12084(B)]
    See all HSD17B8 primary antibodies
  • Description

    Rabbit monoclonal [EPR12084(B)] to HSD17B8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, HepG2, BxPC-3 and JAR cell lysates. JAR cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12084(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis

Associated products

  • Alternative Versions

    • Anti-HSD17B8 antibody [EPR12084(B)] - BSA and Azide free (ab249502)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human HSD17B8 protein (ab105611)
  • Related Products

    • Recombinant Human HSD17B8 protein (ab105611)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/250.
WB
1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
IP
1/10 - 1/100.
Notes
ICC/IF
1/100 - 1/250.
WB
1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt or IHC-P.

Target

  • Function

    NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone. The heteroteramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria.
  • Tissue specificity

    Highly expressed in placenta, liver and pancreas, lower in the skeletal muscle and kidney. Widely expressed.
  • Pathway

    Steroid biosynthesis; estrogen biosynthesis.
    Lipid metabolism; fatty acid biosynthesis.
  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession Q92506 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7923 Human
    • Omim: 601417 Human
    • SwissProt: Q92506 Human
    • Unigene: 415058 Human
    • Alternative names

      • 17 beta HSD 8 antibody
      • 17 beta hydroxysteroid dehydrogenase 8 antibody
      • 17-beta-HSD 8 antibody
      • 17-beta-hydroxysteroid dehydrogenase 8 antibody
      • 17-beta-hydroxysteroid dehydrogenase VIII antibody
      • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
      • 3-oxoacyl-acyl-carrier protein reductase, E. coli, homolog of antibody
      • Beta ketoacyl [acyl carrier protein] reductase like antibody
      • D6S2245E antibody
      • DHB8_HUMAN antibody
      • dJ1033B10.9 antibody
      • Estradiol 17 beta dehydrogenase 8 antibody
      • Estradiol 17-beta-dehydrogenase 8 antibody
      • Estrogen 17 oxidoreductase antibody
      • FABG antibody
      • FabG, E. coli, homolog of antibody
      • FabG-like antibody
      • FABGL antibody
      • H2 KE6 antibody
      • H2-KE6 antibody
      • HKE6 antibody
      • HSD17B8 antibody
      • Hydroxysteroid (17 beta) dehydrogenase 8 antibody
      • Ke-6 antibody
      • KE6 antibody
      • KE6, mouse homolog of antibody
      • Protein Ke6 antibody
      • Really interesting new gene 2 protein antibody
      • RING2 antibody
      • SDR30C1 antibody
      • Short chain dehydrogenase/reductase family 30C member 1 antibody
      • Testosterone 17 beta dehydrogenase 8 antibody
      • Testosterone 17-beta-dehydrogenase 8 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
      Immunocytochemistry/ Immunofluorescence - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
      Immunofluorescent analysis of JAR cells labelling HSD17B8 with ab169752 at 1/100 dilution.
    • Western blot - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
      Western blot - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
      All lanes : Anti-HSD17B8 antibody [EPR12084(B)] (ab169752) at 1/10000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Bx-PC-3 lysate
      Lane 4 : JAR lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 26 kDa

    • Immunoprecipitation - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
      Immunoprecipitation - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
      Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate labeling HSD17B8 with ab169752 at 1/10 dilution or (2) 1X PBS (negative control. HRP-conjugated anti-rabbit IgG preferentially detected the non-reduced form of Rabbit IgG.
    • Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
      Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab169752? Please let us know so that we can cite the reference in this datasheet.

    ab169752 has not yet been referenced specifically in any publications.

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