Recombinant Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12084(B)] to HSD17B8
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
Overview
-
Product name
Anti-HSD17B8 antibody [EPR12084(B)]
See all HSD17B8 primary antibodies -
Description
Rabbit monoclonal [EPR12084(B)] to HSD17B8 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to amino acid residues in Human HSD17B8 (Q92506).
-
Positive control
- Human fetal liver, HepG2, BxPC-3 and JAR cell lysates. JAR cells.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12084(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab169752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/10000 - 1/50000. Predicted molecular weight: 26 kDa. | |
| IP | 1/10 - 1/100. |
Target
-
Function
NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone. The heteroteramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria. -
Tissue specificity
Highly expressed in placenta, liver and pancreas, lower in the skeletal muscle and kidney. Widely expressed. -
Pathway
Steroid biosynthesis; estrogen biosynthesis.
Lipid metabolism; fatty acid biosynthesis. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 7923 Human
- Omim: 601417 Human
- SwissProt: Q92506 Human
- Unigene: 415058 Human
-
Alternative names
- 17 beta HSD 8 antibody
- 17 beta hydroxysteroid dehydrogenase 8 antibody
- 17-beta-HSD 8 antibody
see all
Images
-
![Immunocytochemistry/ Immunofluorescence - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)](https://www.abcam.com/ps/products/169/ab169752/Images/HSD17B8-Primary-antibodies-ab169752-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)Immunofluorescent analysis of JAR cells labelling HSD17B8 with ab169752 at 1/100 dilution. -
![Western blot - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)](https://www.abcam.com/ps/products/169/ab169752/Images/HSD17B8-Primary-antibodies-ab169752-2.jpg)
Western blot - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)All lanes : Anti-HSD17B8 antibody [EPR12084(B)] (ab169752) at 1/10000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : Bx-PC-3 lysate
Lane 4 : JAR lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Immunoprecipitation - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)](https://www.abcam.com/ps/products/169/ab169752/Images/HSD17B8-Primary-antibodies-ab169752-3.jpg)
Immunoprecipitation - Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate labeling HSD17B8 with ab169752 at 1/10 dilution or (2) 1X PBS (negative control. HRP-conjugated anti-rabbit IgG preferentially detected the non-reduced form of Rabbit IgG. -
![Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)](https://www.abcam.com/ps/products/169/ab169752/Images/ab169752-4-benefits-of-recombinant-antibodies.png)
Anti-HSD17B8 antibody [EPR12084(B)] (ab169752)
Protocols
Datasheets and documents
References (0)
ab169752 has not yet been referenced specifically in any publications.
