Product nameAnti-HSD17B8 antibody [EPR12085(B)]
See all HSD17B8 primary antibodies
DescriptionRabbit monoclonal [EPR12085(B)] to HSD17B8
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide, corresponding to residues in Human HSD17B8 (UniProt: Q92506).
- Human fetal liver, HepG2, HeLa and Human fetal kidney lysates; Human colon and Human liver tissues.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab169542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 26 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionNAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone. The heteroteramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria.
Tissue specificityHighly expressed in placenta, liver and pancreas, lower in the skeletal muscle and kidney. Widely expressed.
PathwaySteroid biosynthesis; estrogen biosynthesis.
Lipid metabolism; fatty acid biosynthesis.
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- 17 beta HSD 8 antibody
- 17 beta hydroxysteroid dehydrogenase 8 antibody
- 17-beta-HSD 8 antibody
All lanes : Anti-HSD17B8 antibody [EPR12085(B)] (ab169542) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : HeLa lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling HSD17B8 with ab169542 antibody at 1/50.
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling HSD17B8 with ab169542 antibody at 1/50.
ab169542 has not yet been referenced specifically in any publications.