• Product name
  • Description
    Mouse monoclonal to HSD3B1
  • Host species
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human HSD3B1 aa 1-373.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab167417 as a replacement.



Our Abpromise guarantee covers the use of ab55268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 21094146


  • Function
    3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.
  • Tissue specificity
    Placenta and skin. Predominantly expressed in mammary gland tissue.
  • Pathway
    Lipid metabolism; steroid biosynthesis.
  • Sequence similarities
    Belongs to the 3-beta-HSD family.
  • Cellular localization
    Endoplasmic reticulum membrane. Mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 beta and steroid delta isomerase 1 antibody
    • 3 beta and steroid hydroxy delta 5 steroid dehydrogenase antibody
    • 3 beta HSD I antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I antibody
    • 3-beta-HSD I antibody
    • 3-beta-hydroxy-5-ene steroid dehydrogenase antibody
    • 3BETAHSD antibody
    • 3BH antibody
    • 3BHS1_HUMAN antibody
    • 3BHSD antibody
    • Delta-5-3-ketosteroid isomerase antibody
    • HGNC:5217 antibody
    • HSD3B antibody
    • HSD3B1 antibody
    • HSDB3 antibody
    • HSDB3A antibody
    • Hydroxy delta 5 steroid dehydrogenase 3 beta and steroid delta isomerase 1 antibody
    • Hydroxy delta 5 steroid dehydrogenase antibody
    • Progesterone reductase antibody
    • SDR11E1 antibody
    • Steroid Delta-isomerase antibody
    • trophoblast antigen antibody
    • Trophoblast antigen FDO161G antibody
    see all


  • HSD3B1 antibody (ab55268) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human placenta.
  • Anti-HSD3B1 antibody (ab55268) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa
    Additional bands at: 73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 4 minutes
  • Overlay histogram showing JEG3 cells stained with ab55268 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55268, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in JEG3 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.


This product has been referenced in:
  • Xie T  et al. Roscovitine protects murine Leydig cells from lipopolysaccharide-induced inflammation. Exp Ther Med 13:2169-2176 (2017). Read more (PubMed: 28565824) »
  • Hagberg Thulin M  et al. Osteoblasts promote castration-resistant prostate cancer by altering intratumoral steroidogenesis. Mol Cell Endocrinol 422:182-191 (2016). WB, IHC-P . Read more (PubMed: 26586211) »

See all 7 Publications for this product

Customer reviews and Q&As

Anti-HSD3B1 antibody (ab55268) is raised against the full length protein, corresponding to amino acids 1-374 of Human HSD3B1.

Sequence alignment of Human HSD3B1 against HSD3B2 is 94%.

This suggests that ab55268 is not specific to HSD...

Read More

Thank you for your reply.

We recommend using 5% milk or BSA for the blocking step, however, when incubating with the primary antibody we recommend reducing that concentration to 1% or less. Blocking agent is not strictly necessary in the anti...

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Thank you for contacting us and for forwarding the customer's protocol information. I am sorry that this antibody is not producing satisfactory results in WB. Could you please provide some additional information about the experiment?

1) Were ...

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Thank you for filling in the questionnaire and for reporting this problem to us. We will use the details you sent about your protocol in order to investigate the source of the problem. I have looked through the protocol, which looks fine to me....

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Thank you for contacting us. The HSD3B1 protein has 71% similarity between human and mouse. Theoretically the antibody should not be detecting the mouse protein however because we have not tested this so will not be able to confirm it. http://w...

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Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Rhesus monkey Tissue sections (Testis)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Placenta)
Antigen retrieval step

Abcam user community

Verified customer

Submitted Jul 20 2009


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