Overview

  • Product name

  • Description

    Rabbit polyclonal to HSD3B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (Mouse)

Properties

Applications

Our Abpromise guarantee covers the use of ab65156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Predicted molecular weight: 42 kDa.

Target

  • Function

    3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.
  • Tissue specificity

    Placenta and skin. Predominantly expressed in mammary gland tissue.
  • Pathway

    Lipid metabolism; steroid biosynthesis.
  • Sequence similarities

    Belongs to the 3-beta-HSD family.
  • Cellular localization

    Endoplasmic reticulum membrane. Mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 beta and steroid delta isomerase 1 antibody
    • 3 beta and steroid hydroxy delta 5 steroid dehydrogenase antibody
    • 3 beta HSD I antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I antibody
    • 3-beta-HSD I antibody
    • 3-beta-hydroxy-5-ene steroid dehydrogenase antibody
    • 3BETAHSD antibody
    • 3BH antibody
    • 3BHS1_HUMAN antibody
    • 3BHSD antibody
    • Delta-5-3-ketosteroid isomerase antibody
    • HGNC:5217 antibody
    • HSD3B antibody
    • HSD3B1 antibody
    • HSDB3 antibody
    • HSDB3A antibody
    • Hydroxy delta 5 steroid dehydrogenase 3 beta and steroid delta isomerase 1 antibody
    • Hydroxy delta 5 steroid dehydrogenase antibody
    • Progesterone reductase antibody
    • SDR11E1 antibody
    • Steroid Delta-isomerase antibody
    • trophoblast antigen antibody
    • Trophoblast antigen FDO161G antibody
    see all

References

This product has been referenced in:

  • Farina M  et al. Transcutaneously refillable, 3D-printed biopolymeric encapsulation system for the transplantation of endocrine cells. Biomaterials 177:125-138 (2018). Read more (PubMed: 29886385) »
  • Guo X  et al. Induced pluripotent stem cell-derived conditional medium promotes Leydig cell anti-apoptosis and proliferation via autophagy and Wnt/ß-catenin pathway. J Cell Mol Med 22:3614-3626 (2018). Read more (PubMed: 29667777) »
See all 9 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis)
Permeabilization
No
Specification
Testis
Blocking step
SuperBlock™ (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Bouin Soluion

Dr. Kuo-Ting Chang

Verified customer

Submitted Nov 28 2017

Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. Reviewing this case, I would like to offer some suggestions to help optimise the results from ab65156. I would also appreciate if you can confirm some further details: 1. I can suggest that as HSD3B1 is an intracellular protein to try including a a permeabilization step. I can recommend trying 0.2% Triton for 10 minutes only. This will allow the antibody to access the target protein. 2. I can recommend including 0.2% Tween (a more gentle detergent) in the wash buffer, antibody dilution buffer and blocking buffer. This will help to keep the cells permeabilized and wash away any excess antibody. Wash 4 times for 5 minutes at the wash steps. 3. I can suggest to try a lower dilution of antibody, 1:200 or even 1:500 overnight 4oC. 4. Could you confirm if the current secondary antibody and detection kit working well with other primary antibodies? What was the result of the non primary control? In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

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