Recombinant Anti-HSD3B1 antibody [EPR9687] (ab167417)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9687] to HSD3B1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-HSD3B1 antibody [EPR9687]
See all HSD3B1 primary antibodies -
Description
Rabbit monoclonal [EPR9687] to HSD3B1 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 83% homology and 6 continuous identical amino acids with HSD3B2(SwissProt: P26439). Cross-reactivity with this protein has not been confirmed experimentally.
-
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human placenta cell lysate, Caco-2 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9687 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
|
|
ICC/IF |
1/250 - 1/500.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
ICC/IF
1/250 - 1/500. |
Target
-
Function
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone. -
Tissue specificity
Placenta and skin. Predominantly expressed in mammary gland tissue. -
Pathway
Lipid metabolism; steroid biosynthesis. -
Sequence similarities
Belongs to the 3-beta-HSD family. -
Cellular localization
Endoplasmic reticulum membrane. Mitochondrion membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3283 Human
- Entrez Gene: 3284 Human
- Omim: 109715 Human
- SwissProt: P14060 Human
- SwissProt: P26439 Human
- Unigene: 364941 Human
- Unigene: 654399 Human
-
Alternative names
- 3 beta and steroid delta isomerase 1 antibody
- 3 beta and steroid hydroxy delta 5 steroid dehydrogenase antibody
- 3 beta HSD I antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab167417 has been referenced in 4 publications.
- Fraichard C et al. Protease Inhibitor Anti-HIV, Lopinavir, Impairs Placental Endocrine Function. Int J Mol Sci 22:N/A (2021). PubMed: 33445576
- E Silva PV et al. Effects of isolated or combined exposure to sibutramine and rosuvastatin on reproductive parameters of adult male rats. J Appl Toxicol 40:947-964 (2020). PubMed: 32072669
- Nedder M et al. Uptake of Cerium Dioxide Nanoparticles and Impact on Viability, Differentiation and Functions of Primary Trophoblast Cells from Human Placenta. Nanomaterials (Basel) 10:N/A (2020). PubMed: 32635405
- Fraichard C et al. Placental production of progestins is fully effective in villous cytotrophoblasts and increases with the syncytiotrophoblast formation. Mol Cell Endocrinol 499:110586 (2020). PubMed: 31539598