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Rabbit monoclonal [EPR9687] to HSD3B1
ICC/IF more details Unsuitable for:
IHC-P or IP
Synthetic peptide corresponding to residues in Human HSD3B1 (UniProt: P14060)
Human placenta cell lysate, Caco-2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
1/250 - 1/500.
Is unsuitable for IHC-P or IP.
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.
Placenta and skin. Predominantly expressed in mammary gland tissue.
Lipid metabolism; steroid biosynthesis.
Belongs to the 3-beta-HSD family.
Endoplasmic reticulum membrane. Mitochondrion membrane.
Information by UniProt
3 beta and steroid delta isomerase 1 antibody
3 beta and steroid hydroxy delta 5 steroid dehydrogenase antibody
3 beta HSD I antibody
Western blot - Anti-HSD3B1 antibody [EPR9687] (ab167417)
Anti-HSD3B1 antibody [EPR9687] (ab167417) at 1/1000 dilution + Human placenta cell lysate at 10 µg
Secondary Goat anti-rabbit HRP antibody at 1/2000 dilution Predicted band size: 42 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HSD3B1 antibody [EPR9687] (ab167417)
Immunofluorescent analysis of Caco-2 cells labeling HSD3B1 with ab167417 at 1/250 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"