• Product name

    Anti-HSD3B2 antibody [373CT9.1.3]
    See all HSD3B2 primary antibodies
  • Description

    Mouse monoclonal [373CT9.1.3] to HSD3B2
  • Host species

  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Goat, Horse, Cow, Cat, Dog, Pig, Cynomolgus monkey, Macaque monkey, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human HSD3B2 aa 1-100 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • This antibody gave a positive signal in human adrenal tissue lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab75710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa). Abcam recommends blocking using 3% milk.
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Function

    3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
  • Tissue specificity

    Expressed in adrenal gland, testis and ovary.
  • Pathway

    Lipid metabolism; steroid biosynthesis.
  • Involvement in disease

    Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2) [MIM:201810]. AH2 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late onset (NC or LOAH), and 'cryptic' (asymptomatic). In AH2, virilization is much less marked or does not occur. AH2 is frequently lethal in early life.
    Note=Mild HSD3B2 deficiency in hyperandrogenic females is associated with characteristic traits of polycystic ovary syndrome, such as insulin resistance and luteinizing hormon hypersecretion.
  • Sequence similarities

    Belongs to the 3-beta-HSD family.
  • Cellular localization

    Endoplasmic reticulum membrane. Mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 beta HSD adrenal and gonadal type antibody
    • 3 beta HSD II antibody
    • 3 beta HSD type II antibody
    • 3 beta hydroxy 5 ene steroid dehydrogenase antibody
    • 3 beta hydroxy Delta(5) steroid dehydrogenase antibody
    • 3 beta hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • 3-beta-HSD II antibody
    • 3-beta-hydroxy-5-ene steroid dehydrogenase antibody
    • 3-beta-hydroxy-Delta(5)-steroid dehydrogenase antibody
    • 3BHS2_HUMAN antibody
    • beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • delta 5 delta 4 isomerase type II antibody
    • Delta-5-3-ketosteroid isomerase antibody
    • HSD3B antibody
    • HSD3B2 antibody
    • HSDB antibody
    • HSDB3B antibody
    • hydroxy delta 5 steroid dehydrogenase, 3 beta and steroid delta isomerase 2 antibody
    • Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 antibody
    • Progesterone reductase antibody
    • SDR11E2 antibody
    • short chain dehydrogenase/reductase family 11E, member 2 antibody
    • Steroid Delta-isomerase antibody
    see all


  • Anti-HSD3B2 antibody [373CT9.1.3] (ab75710) at 1 µg/ml + Human adrenal normal tissue lysate - total protein (ab29249) at 20 µg

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 110 kDa, 35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 20 minutes

    Blocked using 3% milk.
  • Overlay histogram showing JEG3 cells stained with ab75710 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75710, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


This product has been referenced in:

  • Maoduo Z  et al. Effects of LPS on the accumulation of lipid droplets, proliferation, and steroidogenesis in goat luteinized granulosa cells. J Biochem Mol Toxicol N/A:e22329 (2019). Read more (PubMed: 30934154) »
  • Ide H  et al. Modulation of AKR1C2 by curcumin decreases testosterone production in prostate cancer. Cancer Sci 109:1230-1238 (2018). WB ; Human . Read more (PubMed: 29369461) »
See all 7 Publications for this product

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