Key features and details
- Rabbit polyclonal to HSD3B2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HSD3B2 antibody
See all HSD3B2 primary antibodies
DescriptionRabbit polyclonal to HSD3B2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 126-372 of Human HSD3B2 (UniProt: P26439).
- Raji and HeLaS3 whole cell lysates; HeLa cells; Human A549 xenograft tissue.
HSD3B2 and HSD3B1 proteins are quite similar in sequence so this product may react with both proteins.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab154385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
Function3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Tissue specificityExpressed in adrenal gland, testis and ovary.
PathwayLipid metabolism; steroid biosynthesis.
Involvement in diseaseDefects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2) [MIM:201810]. AH2 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late onset (NC or LOAH), and 'cryptic' (asymptomatic). In AH2, virilization is much less marked or does not occur. AH2 is frequently lethal in early life.
Note=Mild HSD3B2 deficiency in hyperandrogenic females is associated with characteristic traits of polycystic ovary syndrome, such as insulin resistance and luteinizing hormon hypersecretion.
Sequence similaritiesBelongs to the 3-beta-HSD family.
Cellular localizationEndoplasmic reticulum membrane. Mitochondrion membrane.
- Information by UniProt
- 3 beta HSD adrenal and gonadal type antibody
- 3 beta HSD II antibody
- 3 beta HSD type II antibody
Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling HSD3B2 with ab154385 at 1/100 dilution.
All lanes : Anti-HSD3B2 antibody (ab154385) at 1/1000 dilution
Lane 1 : Raji whole cell lysate
Lane 2 : HeLaS3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
10% SDS PAGE
Immunofluorescent analysis of methanol-fixed HeLa cells, labeling HSD3B2 with ab154385 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
ab154385 staining HSD3B2 in horse cumulus cells by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% bovine serum for 1 hour at 18°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 2% BSA) for 18 hours at 18°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
ab154385 has been referenced in 3 publications.
- Murakami M et al. In situ metabolomics of aldosterone-producing adenomas. JCI Insight 4:N/A (2019). PubMed: 31484828
- Qin Y et al. Bone Morphogenetic Protein 15 Knockdown Inhibits Porcine Ovarian Follicular Development and Ovulation. Front Cell Dev Biol 7:286 (2019). PubMed: 31803742
- Xing X et al. Differentiation of human umbilical cord mesenchymal stem cells into steroidogenic cells in vitro. Exp Ther Med 12:3527-3534 (2016). WB, IF . PubMed: 28105086