Overview

  • Product name

  • Description

    Rabbit polyclonal to HSD3B2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Horse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 126-372 of Human HSD3B2 (UniProt: P26439).

  • Positive control

    • Raji and HeLaS3 whole cell lysates; HeLa cells; Human A549 xenograft tissue.
  • General notes

    HSD3B2 and HSD3B1 proteins are quite similar in sequence so this product may react with both proteins.

Properties

Applications

Our Abpromise guarantee covers the use of ab154385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 42 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
  • Tissue specificity

    Expressed in adrenal gland, testis and ovary.
  • Pathway

    Lipid metabolism; steroid biosynthesis.
  • Involvement in disease

    Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2) [MIM:201810]. AH2 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late onset (NC or LOAH), and 'cryptic' (asymptomatic). In AH2, virilization is much less marked or does not occur. AH2 is frequently lethal in early life.
    Note=Mild HSD3B2 deficiency in hyperandrogenic females is associated with characteristic traits of polycystic ovary syndrome, such as insulin resistance and luteinizing hormon hypersecretion.
  • Sequence similarities

    Belongs to the 3-beta-HSD family.
  • Cellular localization

    Endoplasmic reticulum membrane. Mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 beta HSD adrenal and gonadal type antibody
    • 3 beta HSD II antibody
    • 3 beta HSD type II antibody
    • 3 beta hydroxy 5 ene steroid dehydrogenase antibody
    • 3 beta hydroxy Delta(5) steroid dehydrogenase antibody
    • 3 beta hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • 3-beta-HSD II antibody
    • 3-beta-hydroxy-5-ene steroid dehydrogenase antibody
    • 3-beta-hydroxy-Delta(5)-steroid dehydrogenase antibody
    • 3BHS2_HUMAN antibody
    • ADRENAL HYPERPLASIA II antibody
    • beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • delta 5 delta 4 isomerase type II antibody
    • Delta-5-3-ketosteroid isomerase antibody
    • HSD3B antibody
    • HSD3B2 antibody
    • HSDB antibody
    • HSDB3B antibody
    • hydroxy delta 5 steroid dehydrogenase, 3 beta and steroid delta isomerase 2 antibody
    • Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 antibody
    • Progesterone reductase antibody
    • SDR11E2 antibody
    • short chain dehydrogenase/reductase family 11E, member 2 antibody
    • Steroid Delta-isomerase antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling HSD3B2 with ab154385 at 1/100 dilution.
  • All lanes : Anti-HSD3B2 antibody (ab154385) at 1/1000 dilution

    Lane 1 : Raji whole cell lysate
    Lane 2 : HeLaS3 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 42 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells, labeling HSD3B2 with ab154385 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • ab154385 staining HSD3B2 in horse cumulus cells by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% bovine serum for 1 hour at 18°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 2% BSA) for 18 hours at 18°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Xing X  et al. Differentiation of human umbilical cord mesenchymal stem cells into steroidogenic cells in vitro. Exp Ther Med 12:3527-3534 (2016). WB, IF . Read more (PubMed: 28105086) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 18°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1:10 citrate-based solution (pH 6 Antigen Retrieval Citra solution, Biogenex, Fremont, USA)
Sample
Horse Tissue sections (cumulus cells)
Specification
cumulus cells
Permeabilization
No
Fixative
Formaldehyde

Ms. Hilde Nelis

Verified customer

Submitted May 28 2014

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