Key features and details
- Mouse monoclonal [2A11BG6AH6] to HSDL2
- Suitable for: ICC, IP, In-Cell ELISA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-HSDL2 antibody [2A11BG6AH6]
See all HSDL2 primary antibodies
DescriptionMouse monoclonal [2A11BG6AH6] to HSDL2
Tested applicationsSuitable for: ICC, IP, In-Cell ELISAmore details
Species reactivityReacts with: Human
Other Immunogen Type. This information is considered to be commercially sensitive.
- Hela cell and human liver mitochondria.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesPurity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation
Light chain typekappa
Our Abpromise guarantee covers the use of ab110298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 10 µg/ml.|
|IP||Use at an assay dependent dilution.|
|In-Cell ELISA||Use a concentration of 0.4 µg/ml. (0.4 µg/well)|
FunctionHas apparently no steroid dehydrogenase activity.
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 SCP2 domain.
- Information by UniProt
- C9orf99 antibody
- FLJ25855 antibody
- hsdl2 antibody
As an IP antibody, HSDL2 antibody [2A11BG6AH6 ] (ab110298) works with human sample only.
Immunocytochemistry image of HSDL2 stained human Hela cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with HSDL2 antibody [2A11BG6AH6 ](ab110298) at 10 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps.
ab110298 has not yet been referenced specifically in any publications.