1/400. Use with paraformaldehyde or methanol-fixed cells.
Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
Ab70378 (10 µg) was used to immunoprecipitate chromatin from K562 cells. Immunoprecipitated and reference DNA were amplified by ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray and analyzed. As a control a similar experiment was performed using normal rabbit IgG.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for hSET1 using ab70378 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB.
Immunocytochemistry/ Immunofluorescence - Anti-hSET1 antibody (ab70378)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab70378 (1/400) staining hSET1 in assynchronous HeLa cells (green). Cells were paraformaldehyde-fixed, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.
Garcia-Gomez A et al. TET2- and TDG-mediated changes are required for the acquisition of distinct histone modifications in divergent terminal differentiation of myeloid cells. Nucleic Acids Res45:10002-10017 (2017).
Read more (PubMed: 28973458) »
Franks TM et al. Nup98 recruits the Wdr82-Set1A/COMPASS complex to promoters to regulate H3K4 trimethylation in hematopoietic progenitor cells. Genes Dev31:2222-2234 (2017).
Read more (PubMed: 29269482) »