Key features and details
- Rabbit polyclonal to hSET1/SET1
- Suitable for: IHC-P, ChIP/Chip, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-hSET1/SET1 antibody
See all hSET1/SET1 primary antibodies
DescriptionRabbit polyclonal to hSET1/SET1
Tested applicationsSuitable for: IHC-P, ChIP/Chip, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human hSET1/SET1.
Database link: O15047
This product was previously labelled as hSET1
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab70378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ChIP/Chip||Use 10 µg for µg of chromatin.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 186 kDa.|
|IP||Use at 1-4 µg/mg of lysate.|
FunctionHistone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
Sequence similaritiesContains 1 post-SET domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SET domain.
Cellular localizationNucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
- Information by UniProt
- Histone-lysine N-methyltransferase SETD1A antibody
- hSET1A antibody
- KMT2F antibody
Lanes 1-4 : Anti-hSET1/SET1 antibody (ab70378) at 1 µg/ml
Lanes 5-8 : Anti-hSET1/SET1 antibody (ab70378) at 0.2 µg/ml
Lanes 1 & 5 : 293T whole cell lysate at 100 µg
Lanes 2 & 6 : 293T whole cell lysate at 200 µg
Lanes 3 & 7 : 293T nuclear extract at 100 µg
Lanes 4 & 8 : 293T nuclear extract at 200 µg
Developed using the ECL technique.
Predicted band size: 186 kDa
Ab70378 (10 µg) was used to immunoprecipitate chromatin from K562 cells. Immunoprecipitated and reference DNA were amplified by ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray and analyzed. As a control a similar experiment was performed using normal rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling hSET1/SET1 with ab70378 at 1/1000 (1µg/ml). Detection: DAB.
hSET1/SET1 was immunoprecipitated from 293T nuclear extracts with 6 µg/mg lysate ab70378. Western blot was performed from the immunoprecipitate using ab70378 at 0.4 µg/ml.
Lane 1: ab70378 IP in 293T nuclear extract.
Lane 2: Control IgG IP in 293T nuclear extract.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for hSET1/SET1 using ab70378 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB.
ab70378 has been referenced in 11 publications.
- Hoshii T et al. A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response. Cell 172:1007-1021.e17 (2018). PubMed: 29474905
- Garcia-Gomez A et al. TET2- and TDG-mediated changes are required for the acquisition of distinct histone modifications in divergent terminal differentiation of myeloid cells. Nucleic Acids Res 45:10002-10017 (2017). PubMed: 28973458
- Franks TM et al. Nup98 recruits the Wdr82-Set1A/COMPASS complex to promoters to regulate H3K4 trimethylation in hematopoietic progenitor cells. Genes Dev 31:2222-2234 (2017). PubMed: 29269482
- Zhu Y et al. Association Between Histone Methyltransferase hSETD1A and Prognosis in Patients With Triple-Negative Breast Cancer After Surgery: A Retrospective Study in the Chinese Female Population. Medicine (Baltimore) 95:e3783 (2016). IHC . PubMed: 27227949
- Jakobsson ME et al. Correspondence: On the enzymology and significance of HSPA1 lysine methylation. Nat Commun 7:11464 (2016). WB . PubMed: 27321414