Product nameAnti-hSET1/SET1 antibody
See all hSET1/SET1 primary antibodies
DescriptionRabbit polyclonal to hSET1/SET1
Tested applicationsSuitable for: IHC-P, ChIP, ChIP/Chip, WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human hSET1/SET1.
Database link: O15047
- WB: 293T whole cell lysate. IHC-P: Human breast cancer and ovarian carcinoma tissue.
This product was previously labelled as hSET1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab70378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ChIP||Use 1-5 µg for µg of chromatin.|
|ChIP/Chip||Use 10 µg for µg of chromatin.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 186 kDa.|
|IP||Use at 1-4 µg/mg of lysate.|
|ICC/IF||1/400. Use with paraformaldehyde or methanol-fixed cells.|
FunctionHistone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
Sequence similaritiesContains 1 post-SET domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SET domain.
Cellular localizationNucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
- Information by UniProt
- Histone-lysine N-methyltransferase SETD1A antibody
- hSET1A antibody
- KMT2F antibody
Lanes 1-4 : Anti-hSET1/SET1 antibody (ab70378) at 1 µg/ml
Lanes 5-8 : Anti-hSET1/SET1 antibody (ab70378) at 0.2 µg/ml
Lanes 1 & 5 : 293T whole cell lysate at 100 µg
Lanes 2 & 6 : 293T whole cell lysate at 200 µg
Lanes 3 & 7 : 293T nuclear extract at 100 µg
Lanes 4 & 8 : 293T nuclear extract at 200 µg
Developed using the ECL technique.
Predicted band size: 186 kDa
Ab70378 (10 µg) was used to immunoprecipitate chromatin from K562 cells. Immunoprecipitated and reference DNA were amplified by ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray and analyzed. As a control a similar experiment was performed using normal rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling hSET1/SET1 with ab70378 at 1/1000 (1µg/ml). Detection: DAB.
hSET1/SET1 was immunoprecipitated from 293T nuclear extracts with 6 µg/mg lysate ab70378. Western blot was performed from the immunoprecipitate using ab70378 at 0.4 µg/ml.
Lane 1: ab70378 IP in 293T nuclear extract.
Lane 2: Control IgG IP in 293T nuclear extract.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for hSET1/SET1 using ab70378 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB.
ab70378 (1/400) staining hSET1/SET1 in assynchronous HeLa cells (green). Cells were paraformaldehyde-fixed, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.
This product has been referenced in:
- Hoshii T et al. A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response. Cell 172:1007-1021.e17 (2018). Read more (PubMed: 29474905) »
- Garcia-Gomez A et al. TET2- and TDG-mediated changes are required for the acquisition of distinct histone modifications in divergent terminal differentiation of myeloid cells. Nucleic Acids Res 45:10002-10017 (2017). Read more (PubMed: 28973458) »