Overview

  • Product name

  • Description

    Rabbit polyclonal to hSET1/SET1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ChIP, ChIP/Chip, WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human hSET1/SET1.
    Database link: O15047

  • Positive control

    • WB: 293T whole cell lysate. IHC-P: Human breast cancer and ovarian carcinoma tissue.
  • General notes

     This product was previously labelled as hSET1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIP Use 1-5 µg for µg of chromatin.
ChIP/Chip Use 10 µg for µg of chromatin.
WB 1/1000 - 1/10000. Predicted molecular weight: 186 kDa.
IP Use at 1-4 µg/mg of lysate.
ICC/IF 1/400. Use with paraformaldehyde or methanol-fixed cells.

Target

  • Function

    Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
  • Sequence similarities

    Contains 1 post-SET domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SET domain.
  • Cellular localization

    Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Histone-lysine N-methyltransferase SETD1A antibody
    • hSET1A antibody
    • KMT2F antibody
    • Lysine N methyltransferase 2F antibody
    • Lysine N-methyltransferase 2F antibody
    • SET domain containing 1A antibody
    • SET domain containing protein 1A antibody
    • SET domain-containing protein 1A antibody
    • SET1 antibody
    • Set1 Ash2 histone methyltransferase complex subunit SET1 antibody
    • Set1/Ash2 histone methyltransferase complex subunit SET1 antibody
    • SET1A antibody
    • SET1A_HUMAN antibody
    • SETD1A antibody
    see all

Images

  • Lanes 1-4 : Anti-hSET1/SET1 antibody (ab70378) at 1 µg/ml
    Lanes 5-8 : Anti-hSET1/SET1 antibody (ab70378) at 0.2 µg/ml

    Lanes 1 & 5 : 293T whole cell lysate at 100 µg
    Lanes 2 & 6 : 293T whole cell lysate at 200 µg
    Lanes 3 & 7 : 293T nuclear extract at 100 µg
    Lanes 4 & 8 : 293T nuclear extract at 200 µg

    Developed using the ECL technique.

    Predicted band size: 186 kDa

  • Ab70378 (10 µg) was used to immunoprecipitate chromatin from K562 cells. Immunoprecipitated and reference DNA were amplified by ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray and analyzed. As a control a similar experiment was performed using normal rabbit IgG.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling hSET1/SET1 with ab70378 at 1/1000 (1µg/ml). Detection: DAB.

  • hSET1/SET1 was immunoprecipitated from 293T nuclear extracts with 6 µg/mg lysate ab70378. Western blot was performed from the immunoprecipitate using ab70378 at 0.4 µg/ml.

    Lane 1: ab70378 IP in 293T nuclear extract.

    Lane 2: Control IgG IP in 293T nuclear extract.

  • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for hSET1/SET1 using ab70378 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB.

  • ab70378 (1/400) staining hSET1/SET1 in assynchronous HeLa cells (green). Cells were paraformaldehyde-fixed, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

References

This product has been referenced in:

  • Hoshii T  et al. A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response. Cell 172:1007-1021.e17 (2018). Read more (PubMed: 29474905) »
  • Garcia-Gomez A  et al. TET2- and TDG-mediated changes are required for the acquisition of distinct histone modifications in divergent terminal differentiation of myeloid cells. Nucleic Acids Res 45:10002-10017 (2017). Read more (PubMed: 28973458) »
See all 10 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Gonad)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Gonad
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 17 2014

Application
Western blot
Sample
Mouse Cell lysate - nuclear (gonadotrope)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
20 µg
Specification
gonadotrope
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Andrea Wijeweera

Verified customer

Submitted Nov 11 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 15 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up