Overview

  • Product name

  • Description

    Rabbit polyclonal to hSET1/SET1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human hSET1/SET1 (C terminal).

  • Positive control

    • 293T whole cell lysate or nuclear extracts
  • General notes

     This product was previously labelled as hSET1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab70379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 186 kDa.
IP Use at 1-4 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration. PubMed: 22067449

Target

  • Function

    Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
  • Sequence similarities

    Contains 1 post-SET domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SET domain.
  • Cellular localization

    Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Histone-lysine N-methyltransferase SETD1A antibody
    • hSET1A antibody
    • KMT2F antibody
    • Lysine N methyltransferase 2F antibody
    • Lysine N-methyltransferase 2F antibody
    • SET domain containing 1A antibody
    • SET domain containing protein 1A antibody
    • SET domain-containing protein 1A antibody
    • SET1 antibody
    • Set1 Ash2 histone methyltransferase complex subunit SET1 antibody
    • Set1/Ash2 histone methyltransferase complex subunit SET1 antibody
    • SET1A antibody
    • SET1A_HUMAN antibody
    • SETD1A antibody
    see all

Images

  • Lanes 1-4 : Anti-hSET1/SET1 antibody (ab70379) at 1 µg/ml
    Lanes 5-8 : Anti-hSET1/SET1 antibody (ab70379) at 0.2 µg/ml

    Lanes 1 & 5 : 293T whole cell lysate at 100 µg
    Lanes 2 & 6 : 293T whole cell lysate at 200 µg
    Lanes 3 & 7 : 293T nuclear lysate at 100 µg
    Lanes 4 & 8 : 293T nuclear lysate at 200 µg

    Developed using the ECL technique.

    Predicted band size: 186 kDa
    Observed band size: >250 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds
  • Detection of Human hSET1/SET1 by Immunoprecipitation. Control IgG used in lane 1, ab70379 used at 5 µg/5mg nuclear extract from 293T cells in lane 2. Immunoprecipitated hSET1/SET1 was detected with post IP WB using ab70379 at 0.2 µg/ml. Detection: Chemiluminescence.

References

This product has been referenced in:

  • Le Douce V  et al. LSD1 cooperates with CTIP2 to promote HIV-1 transcriptional silencing. Nucleic Acids Res 40:1904-15 (2012). ICC/IF, ChIP . Read more (PubMed: 22067449) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3T3)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
40 µg
Specification
NIH3T3
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Apr 20 2012

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