• Product name

  • Description

    Rabbit polyclonal to hSET1/SET1
  • Host species

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human hSET1/SET1 (C terminal).

  • Positive control

    • 293T whole cell lysate or nuclear extracts
  • General notes

     This product was previously labelled as hSET1




Our Abpromise guarantee covers the use of ab70379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 186 kDa.
IP Use at 1-4 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration. PubMed: 22067449


  • Function

    Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
  • Sequence similarities

    Contains 1 post-SET domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SET domain.
  • Cellular localization

    Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Histone-lysine N-methyltransferase SETD1A antibody
    • hSET1A antibody
    • KMT2F antibody
    • Lysine N methyltransferase 2F antibody
    • Lysine N-methyltransferase 2F antibody
    • SET domain containing 1A antibody
    • SET domain containing protein 1A antibody
    • SET domain-containing protein 1A antibody
    • SET1 antibody
    • Set1 Ash2 histone methyltransferase complex subunit SET1 antibody
    • Set1/Ash2 histone methyltransferase complex subunit SET1 antibody
    • SET1A antibody
    • SET1A_HUMAN antibody
    • SETD1A antibody
    see all


  • Lanes 1-4 : Anti-hSET1/SET1 antibody (ab70379) at 1 µg/ml
    Lanes 5-8 : Anti-hSET1/SET1 antibody (ab70379) at 0.2 µg/ml

    Lanes 1 & 5 : 293T whole cell lysate at 100 µg
    Lanes 2 & 6 : 293T whole cell lysate at 200 µg
    Lanes 3 & 7 : 293T nuclear lysate at 100 µg
    Lanes 4 & 8 : 293T nuclear lysate at 200 µg

    Developed using the ECL technique.

    Predicted band size: 186 kDa
    Observed band size: >250 kDa
    why is the actual band size different from the predicted?

    Exposure time: 3 seconds
  • Detection of Human hSET1/SET1 by Immunoprecipitation. Control IgG used in lane 1, ab70379 used at 5 µg/5mg nuclear extract from 293T cells in lane 2. Immunoprecipitated hSET1/SET1 was detected with post IP WB using ab70379 at 0.2 µg/ml. Detection: Chemiluminescence.


This product has been referenced in:

  • Le Douce V  et al. LSD1 cooperates with CTIP2 to promote HIV-1 transcriptional silencing. Nucleic Acids Res 40:1904-15 (2012). ICC/IF, ChIP . Read more (PubMed: 22067449) »
See 1 Publication for this product

Customer reviews and Q&As

Western blot
Mouse Cell lysate - whole cell (NIH3T3)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
40 µg
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

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Submitted Apr 20 2012

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