Key features and details
- Rabbit polyclonal to hSET1/SET1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-hSET1/SET1 antibody
See all hSET1/SET1 primary antibodies
DescriptionRabbit polyclonal to hSET1/SET1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human hSET1/SET1 (C terminal).
This product was previously labelled as hSET1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 186 kDa.|
|IP||Use at 1-4 µg/mg of lysate.|
FunctionHistone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
Sequence similaritiesContains 1 post-SET domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SET domain.
Cellular localizationNucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
- Information by UniProt
- Histone-lysine N-methyltransferase SETD1A antibody
- hSET1A antibody
- KMT2F antibody
Lanes 1-4 : Anti-hSET1/SET1 antibody (ab70379) at 1 µg/ml
Lanes 5-8 : Anti-hSET1/SET1 antibody (ab70379) at 0.2 µg/ml
Lanes 1 & 5 : 293T whole cell lysate at 100 µg
Lanes 2 & 6 : 293T whole cell lysate at 200 µg
Lanes 3 & 7 : 293T nuclear lysate at 100 µg
Lanes 4 & 8 : 293T nuclear lysate at 200 µg
Developed using the ECL technique.
Predicted band size: 186 kDa
Observed band size: >250 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds
Detection of Human hSET1/SET1 by Immunoprecipitation. Control IgG used in lane 1, ab70379 used at 5 µg/5mg nuclear extract from 293T cells in lane 2. Immunoprecipitated hSET1/SET1 was detected with post IP WB using ab70379 at 0.2 µg/ml. Detection: Chemiluminescence.
ab70379 has been referenced in 1 publication.
- Le Douce V et al. LSD1 cooperates with CTIP2 to promote HIV-1 transcriptional silencing. Nucleic Acids Res 40:1904-15 (2012). ICC/IF, ChIP . PubMed: 22067449