Product nameAnti-hSET1/SET1 antibody [BLR024E]
See all hSET1/SET1 primary antibodies
DescriptionRabbit monoclonal [BLR024E] to hSET1/SET1
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human hSET1/SET1 aa 1200-1250. The exact sequence is proprietary. Gene ID 9739.
Database link: O15047
This product is sold under License from Bethyl Laboratories, Inc.
This product was previously labelled as hSET1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.
Use 20µl/mg lysate.
FunctionHistone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
Sequence similaritiesContains 1 post-SET domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SET domain.
Cellular localizationNucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
- Information by UniProt
- Histone-lysine N-methyltransferase SETD1A antibody
- hSET1A antibody
- KMT2F antibody
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for hSET1/SET1 using ab243881 at 1/25 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for hSET1/SET1 using ab243881 at 1/25 dilution in immunohistochemical analysis. A DyLight594-conjugated goat anti-rabbit antibody was used as the secondary. The nuclear counter stain is DAPI (blue)
hSET1/SET1 was immunoprecipitated from 1.0 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab243881 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243881 at 1/1000 dilution.
Lane 1: ab243881 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Western blot analysis using ab243881 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate.
Lane 2: HEK-293T whole cell lysate .
Lane3: Jurkat whole cell lysate.
Lane 4: TCMK-1 whole cell lysate.
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary.
ab243881 has not yet been referenced specifically in any publications.