Overview

  • Product name

    Anti-hSET1/SET1 antibody [BLR024E]
    See all hSET1/SET1 primary antibodies
  • Description

    Rabbit monoclonal [BLR024E] to hSET1/SET1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human hSET1/SET1 aa 1200-1250. The exact sequence is proprietary. Gene ID 9739.
    Database link: O15047

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

     This product was previously labelled as hSET1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 8.2
    Preservative: 0.09% Sodium azide
    Constituents: 98% Borate buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purification notes

    Recombinant antibody was purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    BLR024E
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab243881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IHC-P 1/25 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Use 20µl/mg lysate.

Target

  • Function

    Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
  • Sequence similarities

    Contains 1 post-SET domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SET domain.
  • Cellular localization

    Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Histone-lysine N-methyltransferase SETD1A antibody
    • hSET1A antibody
    • KMT2F antibody
    • Lysine N methyltransferase 2F antibody
    • Lysine N-methyltransferase 2F antibody
    • SET domain containing 1A antibody
    • SET domain containing protein 1A antibody
    • SET domain-containing protein 1A antibody
    • SET1 antibody
    • Set1 Ash2 histone methyltransferase complex subunit SET1 antibody
    • Set1/Ash2 histone methyltransferase complex subunit SET1 antibody
    • SET1A antibody
    • SET1A_HUMAN antibody
    • SETD1A antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for hSET1/SET1 using ab243881 at 1/25 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

  • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for hSET1/SET1 using ab243881 at 1/25 dilution in immunohistochemical analysis. A DyLight594-conjugated goat anti-rabbit antibody was used as the secondary. The nuclear counter stain is DAPI (blue)

  • hSET1/SET1 was immunoprecipitated from 1.0 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab243881 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243881 at 1/1000 dilution.

    Lane 1: ab243881 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

  • Western blot analysis using ab243881 at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate.

    Lane 2: HEK-293T whole cell lysate .

    Lane3: Jurkat whole cell lysate.

    Lane 4: TCMK-1 whole cell lysate.

    A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary.

References

ab243881 has not yet been referenced specifically in any publications.

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