Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR024E] to hSET1/SET1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
Product nameAnti-hSET1/SET1 antibody [BLR024E]
See all hSET1/SET1 primary antibodies
DescriptionRabbit monoclonal [BLR024E] to hSET1/SET1
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human hSET1/SET1 aa 1200-1250. The exact sequence is proprietary. Gene ID 9739.
Database link: O15047
This product is sold under License from Bethyl Laboratories, Inc.
This product was previously labelled as hSET1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.
Use 20µl/mg lysate.
FunctionHistone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
Sequence similaritiesContains 1 post-SET domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SET domain.
Cellular localizationNucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
- Information by UniProt
- Histone-lysine N-methyltransferase SETD1A antibody
- hSET1A antibody
- KMT2F antibody
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for hSET1/SET1 using ab243881 at 1/25 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for hSET1/SET1 using ab243881 at 1/25 dilution in immunohistochemical analysis. A DyLight594-conjugated goat anti-rabbit antibody was used as the secondary. The nuclear counter stain is DAPI (blue)
hSET1/SET1 was immunoprecipitated from 1.0 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab243881 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243881 at 1/1000 dilution.
Lane 1: ab243881 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Western blot analysis using ab243881 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate.
Lane 2: HEK-293T whole cell lysate .
Lane3: Jurkat whole cell lysate.
Lane 4: TCMK-1 whole cell lysate.
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243881 has not yet been referenced specifically in any publications.