Overview

  • Product name

    Anti-HSF1 antibody [4D5F4]
    See all HSF1 primary antibodies
  • Description

    Mouse monoclonal [4D5F4] to HSF1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human HSF1 aa 256-359.
    Sequence:

    DAVASSGPIISDITELAPASPMASPGGSIDERPLSSSPLVRVKEEPPSPP QSPRVEEASPGRPSSVDTLLSPTALIDSILRESEPAPASVTALTDARGHT DTEG


    Database link: Q00613

  • Positive control

    • Human rectum cancer tissue. A431 cells. HSF1 transected HEK293 cell lysates and recombinant HSF1 protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab201978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 57 kDa.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • Sequence similarities

    Belongs to the HSF family.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications

    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt
  • Database links

  • Alternative names

    • Heat shock factor 1 antibody
    • Heat shock factor protein 1 antibody
    • Heat shock transcription factor 1 antibody
    • HSF 1 antibody
    • hsf1 antibody
    • HSF1_HUMAN antibody
    • HSTF 1 antibody
    • HSTF1 antibody
    see all

Images

  • Anti-HSF1 antibody [4D5F4] (ab201978) at 1/500 dilution + recombinant Human HSF1 protein

    Predicted band size: 57 kDa

  • All lanes : Anti-HSF1 antibody [4D5F4] (ab201978) at 1/500 dilution

    Lane 1 : Non-transfected HEK293 cell lysates
    Lane 2 : HSF1 transfected HEK293 cell lysates

    Predicted band size: 57 kDa

  • Immunofluorescence analysis of A431 cells labeling HSF1 using ab201978 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. 

  • Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues labeling HSF1 using ab201978 at 1/200 dilution followed by DAB staining.

References

ab201978 has not yet been referenced specifically in any publications.

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