Product nameAnti-HSF1 (phospho S303 + S307) antibody [EP1711Y]
See all HSF1 primary antibodies
DescriptionRabbit monoclonal [EP1711Y] to HSF1 (phospho S303 + S307)
Specificityab81281 antibody recognises HSF1 (phospho S303 + S307)
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human HSF1 aa 250-350. The exact sequence is proprietary.
- HeLa cell lysates, Human breast carcinoma.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab81281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/50000. Predicted molecular weight: 57 kDa.Can be blocked with Recombinant Human HSF1 protein (ab78795).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
FunctionDNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Sequence similaritiesBelongs to the HSF family.
Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
modificationsPhosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
- Information by UniProt
- Heat shock factor 1 antibody
- Heat shock factor protein 1 antibody
- Heat shock transcription factor 1 antibody
All lanes : Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281) at 1/50000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate AP treated
Lysates/proteins at 10 µg per lane.
All lanes : HRp labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using 1/100 ab81281
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Zhao J et al. Association between integrin-linked kinase and hyperthermia in oral squamous cell carcinoma. Oncol Lett 14:7705-7714 (2017). Read more (PubMed: 29250172) »
- Asano Y et al. IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis. Sci Rep 6:19174 (2016). WB . Read more (PubMed: 26754925) »