Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)
![Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)](https://www.abcam.com/ps/products/81/ab81281/Images/ab81281-96465-anti-hsf1-phospho-s303-s307-antibody-ep1711y-western-blot.jpg "Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)")
Key features and details
- Rabbit monoclonal [EP1711Y] to HSF1 (phospho S303 + S307)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y]
See all HSF1 primary antibodies -
Description
Rabbit monoclonal [EP1711Y] to HSF1 (phospho S303 + S307) -
Host species
Rabbit -
Specificity
ab81281 antibody recognises HSF1 (phospho S303 + S307) -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HSF1 aa 250-350. The exact sequence is proprietary.
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Positive control
- HeLa cell lysates, Human breast carcinoma.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1711Y -
Isotype
IgG -
Research areas
Associated products
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab81281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/50000. Predicted molecular weight: 57 kDa.Can be blocked with Recombinant Human HSF1 protein (ab78795). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. -
Sequence similarities
Belongs to the HSF family. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Post-translational
modificationsPhosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules. - Information by UniProt
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Database links
- Entrez Gene: 3297 Human
- Omim: 140580 Human
- SwissProt: Q00613 Human
- Unigene: 530227 Human
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Alternative names
- Heat shock factor 1 antibody
- Heat shock factor protein 1 antibody
- Heat shock transcription factor 1 antibody
see all
Images
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Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)All lanes : Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281) at 1/50000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate AP treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRp labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 57 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)](https://www.abcam.com/ps/products/81/ab81281/Images/ab81281-96464-anti-hsf1-phospho-s303-s307-antibody-ep1711y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)Immunohistochemical analysis of paraffin-embedded human breast carcinoma using 1/100 ab81281
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (4)
ab81281 has been referenced in 4 publications.
- Hwang HV et al. Impaired proteostasis in senescent vascular endothelial cells: a perspective on estrogen and oxidative stress in the aging vasculature. Am J Physiol Heart Circ Physiol 316:H421-H429 (2019). PubMed: 30499713
- Zhao J et al. Association between integrin-linked kinase and hyperthermia in oral squamous cell carcinoma. Oncol Lett 14:7705-7714 (2017). PubMed: 29250172
- Asano Y et al. IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis. Sci Rep 6:19174 (2016). WB . PubMed: 26754925
- Zhao J et al. Correlation between the expression of Id-1 and hyperthermia-associated molecules in oral squamous cell carcinoma. J Clin Pathol 66:758-63 (2013). IHC ; Human . PubMed: 23723304
