Question (61700) | Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281)

Go to datasheet (ab81281)

Question

Inquiry: this antibody, as written on the datasheet, does not react with mouse tissue and murine species.... ONLY human (also on epitomics website) Lindquist (Cell and JCI...) has published the usage of this ab in mice cell line and tissues..... please could you confirm that ab76076 and ab81281 work on WB on mice cells and mice tissues, thank you best regards

Answer

Thank you for contacting us and bringing this to our attention.


We have tested both products (ab76076 and ab81281) in mouse and rat samples, via WB.
The samples we tested were brain, heart, kidney and spleen for each, in mouse and rat. We only saw a few non-specific bands (with both products) in mouse kidney. No bands were observed in the other lysates.
We do not recommend these products for use in mouse and rat based on this testing.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.