RecombinantRabMAb

Recombinant Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
  • Western blot - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
  • Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1712] to HSF1 (phospho S320) - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free
    See all HSF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1712] to HSF1 (phospho S320) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human HSF1 aa 300-400 (phospho S320). The exact sequence is proprietary.

  • General notes

    ab247451 is the carrier-free version of ab76183 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247451 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab247451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

    • Sequence similarities

      Belongs to the HSF family.

    • Domain

      the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

    • Post-translational
      modifications

      Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
      Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.

    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
    • Information by UniProt

    • Database links

      • Alternative names

        • Heat shock factor 1 antibody
        • Heat shock factor protein 1 antibody
        • Heat shock transcription factor 1 antibody
        • HSF 1 antibody
        • hsf1 antibody
        • HSF1_HUMAN antibody
        • HSTF 1 antibody
        • HSTF1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
        This data was developed using ab76183, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human muscles using ab76183 at 1/100 dilution. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
      • Western blot - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
        Western blot - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
        All lanes : Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183) at 1/20000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : HeLa cell lysate, heat treated at 42°C

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit-HRP at 1/1000 dilution

        Developed using the ECL technique.

        Predicted band size: 57 kDa
        Observed band size: 90 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab76183, the same antibody clone in a different buffer formulation.

         

      • Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
        Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab247451? Please let us know so that we can cite the reference in this datasheet.

      ab247451 has not yet been referenced specifically in any publications.

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