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RecombinantRabMAb

Recombinant Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
  • Western blot - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
  • Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1712] to HSF1 (phospho S320) - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free
    See all HSF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1712] to HSF1 (phospho S320) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247451 is the carrier-free version of ab76183.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

  • Sequence similarities

    Belongs to the HSF family.

  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

  • Post-translational
    modifications

    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt

  • Database links

    • Alternative names

      • Heat shock factor 1 antibody
      • Heat shock factor protein 1 antibody
      • Heat shock transcription factor 1 antibody
      • HSF 1 antibody
      • hsf1 antibody
      • HSF1_HUMAN antibody
      • HSTF 1 antibody
      • HSTF1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
      This data was developed using ab76183, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human muscles using ab76183 at 1/100 dilution. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
    • Western blot - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
      Western blot - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
      All lanes : Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate, heat treated at 42°C

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit-HRP at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 57 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?



      This data was developed using ab76183, the same antibody clone in a different buffer formulation.

       

    • Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
      Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247451? Please let us know so that we can cite the reference in this datasheet.

    ab247451 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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