Anti-HSF4 antibody [2E7] (ab128146)
Key features and details
- Mouse monoclonal [2E7] to HSF4
- Suitable for: WB, ELISA, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HSF4 antibody [2E7] -
Description
Mouse monoclonal [2E7] to HSF4 -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified recombinant fragment of human HSF4 expressed in E. Coli.
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Positive control
- Hela cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
contains 0.5% protein stabilizer -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2E7 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab128146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/2000. Predicted molecular weight: 53 kDa. | |
ELISA | 1/10000. | |
Flow Cyt | 1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
DNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription. -
Tissue specificity
Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. -
Involvement in disease
Defects in HSF4 are the cause of cataract zonular HSF4-related (CZ-HSF4) [MIM:116800]. A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes.
Defects in HSF4 are the cause of cataract Marner type (CAM) [MIM:116800]. A form of cataract with variable and progressive opacities. Affected individuals present with zonular cataract, although some have nuclear, anterior polar, or stellate cataract. Finger malformation is observed in some kindreds. -
Sequence similarities
Belongs to the HSF family. -
Post-translational
modificationsPhosphorylated mainly on serine residues. Phosphorylation on Ser-298 promotes sumoylation on Lys-293.
Isoform HSF4B is constitutively sumoylated. Sumoylation represses the transcriptional activity and is promoted by phosphorylation on Ser-298. HSFA is not sumoylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3299 Human
- Omim: 602438 Human
- SwissProt: Q9ULV5 Human
- Unigene: 512156 Human
- Unigene: 710714 Human
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Alternative names
- Cataract, Marner antibody
- CTM antibody
- Heat shock factor protein 4 antibody
see all
Images
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Anti-HSF4 antibody [2E7] (ab128146) at 1/500 dilution + Human HSF4 recombinant protein.
Developed using the ECL technique.
Predicted band size: 53 kDa -
Flow cytometric analysis of HeLa cells using HSF4 mouse mAb (green) and negative control (red).
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Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).
Datasheets and documents
References (0)
ab128146 has not yet been referenced specifically in any publications.