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    hsf4-antibody-2e7-ab128146.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-HSF4 antibody [2E7] (ab128146)

  • Datasheet
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Abpromise

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Western blot - Anti-HSF4 antibody [2E7] (ab128146)
  • Flow Cytometry - Anti-HSF4 antibody [2E7] (ab128146)
  • ELISA - Anti-HSF4 antibody [2E7] (ab128146)

Key features and details

  • Mouse monoclonal [2E7] to HSF4
  • Suitable for: WB, ELISA, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-HSF4 antibody [2E7]
  • Description

    Mouse monoclonal [2E7] to HSF4
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of human HSF4 expressed in E. Coli.

  • Positive control

    • Hela cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    contains 0.5% protein stabilizer
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    2E7
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

Our Abpromise guarantee covers the use of ab128146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 53 kDa.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    DNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription.
  • Tissue specificity

    Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues.
  • Involvement in disease

    Defects in HSF4 are the cause of cataract zonular HSF4-related (CZ-HSF4) [MIM:116800]. A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes.
    Defects in HSF4 are the cause of cataract Marner type (CAM) [MIM:116800]. A form of cataract with variable and progressive opacities. Affected individuals present with zonular cataract, although some have nuclear, anterior polar, or stellate cataract. Finger malformation is observed in some kindreds.
  • Sequence similarities

    Belongs to the HSF family.
  • Post-translational
    modifications

    Phosphorylated mainly on serine residues. Phosphorylation on Ser-298 promotes sumoylation on Lys-293.
    Isoform HSF4B is constitutively sumoylated. Sumoylation represses the transcriptional activity and is promoted by phosphorylation on Ser-298. HSFA is not sumoylated.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9ULV5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3299 Human
    • Omim: 602438 Human
    • SwissProt: Q9ULV5 Human
    • Unigene: 512156 Human
    • Unigene: 710714 Human
    • Alternative names

      • Cataract, Marner antibody
      • CTM antibody
      • Heat shock factor protein 4 antibody
      • Heat shock transcription factor 4 antibody
      • hHSF4 antibody
      • HSF 4 antibody
      • HSF4 antibody
      • HSF4_HUMAN antibody
      • HSTF 4 antibody
      see all

    Images

    • Western blot - Anti-HSF4 antibody [2E7] (ab128146)
      Western blot - Anti-HSF4 antibody [2E7] (ab128146)
      Anti-HSF4 antibody [2E7] (ab128146) at 1/500 dilution + Human HSF4 recombinant protein.

      Developed using the ECL technique.

      Predicted band size: 53 kDa

    • Flow Cytometry - Anti-HSF4 antibody [2E7] (ab128146)
      Flow Cytometry - Anti-HSF4 antibody [2E7] (ab128146)
      Flow cytometric analysis of HeLa cells using HSF4 mouse mAb (green) and negative control (red).
    • ELISA - Anti-HSF4 antibody [2E7] (ab128146)
      ELISA - Anti-HSF4 antibody [2E7] (ab128146)
      Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).

    Protocols

    • Flow cytometry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab128146? Please let us know so that we can cite the reference in this datasheet.

    ab128146 has not yet been referenced specifically in any publications.

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