Key features and details
- Mouse monoclonal [2E7] to HSF4
- Suitable for: WB, ELISA, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-HSF4 antibody [2E7]
DescriptionMouse monoclonal [2E7] to HSF4
Tested applicationsSuitable for: WB, ELISA, Flow Cytmore details
Species reactivityReacts with: Human
Purified recombinant fragment of human HSF4 expressed in E. Coli.
- Hela cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
contains 0.5% protein stabilizer
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab128146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 53 kDa.|
|Flow Cyt||1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionDNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription.
Tissue specificityExpressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues.
Involvement in diseaseDefects in HSF4 are the cause of cataract zonular HSF4-related (CZ-HSF4) [MIM:116800]. A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes.
Defects in HSF4 are the cause of cataract Marner type (CAM) [MIM:116800]. A form of cataract with variable and progressive opacities. Affected individuals present with zonular cataract, although some have nuclear, anterior polar, or stellate cataract. Finger malformation is observed in some kindreds.
Sequence similaritiesBelongs to the HSF family.
modificationsPhosphorylated mainly on serine residues. Phosphorylation on Ser-298 promotes sumoylation on Lys-293.
Isoform HSF4B is constitutively sumoylated. Sumoylation represses the transcriptional activity and is promoted by phosphorylation on Ser-298. HSFA is not sumoylated.
- Information by UniProt
- Cataract, Marner antibody
- CTM antibody
- Heat shock factor protein 4 antibody
Anti-HSF4 antibody [2E7] (ab128146) at 1/500 dilution + Human HSF4 recombinant protein.
Developed using the ECL technique.
Predicted band size: 53 kDa
Flow cytometric analysis of HeLa cells using HSF4 mouse mAb (green) and negative control (red).
Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).
ab128146 has not yet been referenced specifically in any publications.