• Product name
    Anti-HSF4 antibody [EP1719Y]
    See all HSF4 primary antibodies
  • Description
    Rabbit monoclonal [EP1719Y] to HSF4
  • Host species
  • Tested applications
    Suitable for: Flow Cyt, WB, ICC, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HSF4 aa 250-350. The exact sequence is proprietary.

  • Positive control
    • HeLa cell lysate Human muscle
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab53291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/5000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ICC 1/50 - 1/100.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      DNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription.
    • Tissue specificity
      Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues.
    • Involvement in disease
      Defects in HSF4 are the cause of cataract zonular HSF4-related (CZ-HSF4) [MIM:116800]. A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes.
      Defects in HSF4 are the cause of cataract Marner type (CAM) [MIM:116800]. A form of cataract with variable and progressive opacities. Affected individuals present with zonular cataract, although some have nuclear, anterior polar, or stellate cataract. Finger malformation is observed in some kindreds.
    • Sequence similarities
      Belongs to the HSF family.
    • Post-translational
      Phosphorylated mainly on serine residues. Phosphorylation on Ser-298 promotes sumoylation on Lys-293.
      Isoform HSF4B is constitutively sumoylated. Sumoylation represses the transcriptional activity and is promoted by phosphorylation on Ser-298. HSFA is not sumoylated.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Cataract, Marner antibody
      • CTM antibody
      • Heat shock factor protein 4 antibody
      • Heat shock transcription factor 4 antibody
      • hHSF4 antibody
      • HSF 4 antibody
      • HSF4 antibody
      • HSF4_HUMAN antibody
      • HSTF 4 antibody
      see all


    • Anti-HSF4 antibody [EP1719Y] (ab53291) at 1/10000 dilution + HeLa cell lysate at 10 µg

      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 53 kDa
      Observed band size: 53 kDa
      Additional bands at: 120 kDa, 40 kDa. We are unsure as to the identity of these extra bands.

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling HSF4 with unpurified ab53291 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Immunohistochemical analysis of human muscle with ab53291 at 1/50 - 1/100 dilution


    ab53291 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As


    Thank you for contacting Abcam regarding ab53291.

    I have confirmed with the lab that this antibody reacts with the HSF4A isoform only, and the immunogen does not share any significant sequence similarity with HSF4B.

    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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