Product nameAnti-HSF4 antibody [EP1719Y]
See all HSF4 primary antibodies
DescriptionRabbit monoclonal [EP1719Y] to HSF4
Tested applicationsSuitable for: Flow Cyt, WB, ICC, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human HSF4 aa 250-350. The exact sequence is proprietary.
- HeLa cell lysate Human muscle
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab53291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||1/5000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
|ICC||1/50 - 1/100.|
|IHC-P||Use at an assay dependent concentration.|
FunctionDNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription.
Tissue specificityExpressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues.
Involvement in diseaseDefects in HSF4 are the cause of cataract zonular HSF4-related (CZ-HSF4) [MIM:116800]. A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes.
Defects in HSF4 are the cause of cataract Marner type (CAM) [MIM:116800]. A form of cataract with variable and progressive opacities. Affected individuals present with zonular cataract, although some have nuclear, anterior polar, or stellate cataract. Finger malformation is observed in some kindreds.
Sequence similaritiesBelongs to the HSF family.
modificationsPhosphorylated mainly on serine residues. Phosphorylation on Ser-298 promotes sumoylation on Lys-293.
Isoform HSF4B is constitutively sumoylated. Sumoylation represses the transcriptional activity and is promoted by phosphorylation on Ser-298. HSFA is not sumoylated.
- Information by UniProt
- Cataract, Marner antibody
- CTM antibody
- Heat shock factor protein 4 antibody
Anti-HSF4 antibody [EP1719Y] (ab53291) at 1/10000 dilution + HeLa cell lysate at 10 µg
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 120 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling HSF4 with unpurified ab53291 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunohistochemical analysis of human muscle with ab53291 at 1/50 - 1/100 dilution
ab53291 has not yet been referenced specifically in any publications.