Key features and details
- Rabbit polyclonal to Hsp105/HSP110
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Hsp105/HSP110 antibody
See all Hsp105/HSP110 primary antibodies
DescriptionRabbit polyclonal to Hsp105/HSP110
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Hsp105/HSP110 (C terminal).
Database link: NP_006635.2
- Human pancreas tissue; HeLa cell extract; HeLa cells.
This product was previously labelled as Hsp105
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
without Mg2+, Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab166780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 96 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
FunctionPrevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
Tissue specificityHighly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
Sequence similaritiesBelongs to the heat shock protein 70 family.
modificationsPhosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
- Information by UniProt
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All lanes : Anti-Hsp105/HSP110 antibody (ab166780) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with synthesized peptide
Predicted band size: 96 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Hsp105/HSP110 with ab166780 at 5 µg/ml.
Immunofluorescent analysis of HeLa cells labeling Hsp105/HSP110 with ab166780 at 1/100 dilution. The image on the right is treated with the synthesized peptide.
ab166780 has not yet been referenced specifically in any publications.