Anti-Hsp105/HSP110 antibody (ab166780)
Key features and details
- Rabbit polyclonal to Hsp105/HSP110
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp105/HSP110 antibody
See all Hsp105/HSP110 primary antibodies -
Description
Rabbit polyclonal to Hsp105/HSP110 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Hsp105/HSP110 (C terminal).
Database link: NP_006635.2 -
Positive control
- Human pancreas tissue; HeLa cell extract; HeLa cells.
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General notes
This product was previously labelled as Hsp105
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
without Mg2+, Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab166780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 96 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/500. |
Target
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Function
Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities. -
Tissue specificity
Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Post-translational
modificationsPhosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10808 Human
- Entrez Gene: 15505 Mouse
- Omim: 610703 Human
- SwissProt: Q92598 Human
- SwissProt: Q61699 Mouse
- Unigene: 36927 Human
- Unigene: 270681 Mouse
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Alternative names
- Antigen NY CO 25 antibody
- Antigen NY-CO-25 antibody
- DKFZp686M05240 antibody
see all
Images
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Western blot - Anti-Hsp105/HSP110 antibody (ab166780)All lanes : Anti-Hsp105/HSP110 antibody (ab166780) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with synthesized peptide
Predicted band size: 96 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody (ab166780)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Hsp105/HSP110 with ab166780 at 5 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody (ab166780)Immunofluorescent analysis of HeLa cells labeling Hsp105/HSP110 with ab166780 at 1/100 dilution. The image on the right is treated with the synthesized peptide.
Protocols
Datasheets and documents
References (0)
ab166780 has not yet been referenced specifically in any publications.
