Anti-Hsp105/HSP110 antibody (ab166780)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Hsp105/HSP110 antibody
See all Hsp105/HSP110 primary antibodies -
Description
Rabbit polyclonal to Hsp105/HSP110 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Hsp105/HSP110 (C terminal).
Database link: NP_006635.2 -
Positive control
- Human pancreas tissue; HeLa cell extract; HeLa cells.
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General notes
This product was previously labelled as Hsp105
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: without Mg2+, Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab166780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 96 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/500. |
Target
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Function
Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities. -
Tissue specificity
Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Post-translational
modificationsPhosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10808 Human
- Entrez Gene: 15505 Mouse
- Omim: 610703 Human
- SwissProt: Q92598 Human
- SwissProt: Q61699 Mouse
- Unigene: 36927 Human
- Unigene: 270681 Mouse
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Alternative names
- Antigen NY CO 25 antibody
- Antigen NY-CO-25 antibody
- DKFZp686M05240 antibody
see all
Images
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Western blot - Anti-Hsp105/HSP110 antibody (ab166780)All lanes : Anti-Hsp105/HSP110 antibody (ab166780) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa cell extract with synthesized peptide
Predicted band size: 96 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody (ab166780)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Hsp105/HSP110 with ab166780 at 5 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody (ab166780)Immunofluorescent analysis of HeLa cells labeling Hsp105/HSP110 with ab166780 at 1/100 dilution. The image on the right is treated with the synthesized peptide.
Protocols
Datasheets and documents
References
ab166780 has not yet been referenced specifically in any publications.
