Overview

  • Product name

    Anti-Hsp105/HSP110 antibody
    See all Hsp105/HSP110 primary antibodies
  • Description

    Rabbit polyclonal to Hsp105/HSP110
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Hamster
  • Immunogen

    Synthetic peptide corresponding to Hamster Hsp105/HSP110. Sequence identical to human and mouse.
    Database link: Q60446

  • Positive control

    • IHC-P: Mouse backskin.
  • General notes

     This product was previously labelled as Hsp105

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 92,97 kDa).
IHC-P 1/100.

Target

  • Function

    Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
  • Tissue specificity

    Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Post-translational
    modifications

    Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen NY CO 25 antibody
    • Antigen NY-CO-25 antibody
    • DKFZp686M05240 antibody
    • Heat shock 105kD alpha antibody
    • Heat shock 105kD antibody
    • Heat shock 105kD beta antibody
    • Heat shock 105kDa protein 1 antibody
    • Heat shock 105kDa protein antibody
    • Heat shock 105kDa/110kDa protein 1 antibody
    • Heat shock 110 kDa protein antibody
    • Heat shock 110kDa protein antibody
    • Heat shock protein 105 kDa antibody
    • HS105_HUMAN antibody
    • HSP105 antibody
    • HSP105A antibody
    • HSP105B antibody
    • HSP110 antibody
    • HSPH 1 antibody
    • Hsph1 antibody
    • KIAA0201 antibody
    • NY CO 25 antibody
    see all

Images

  • Bouin’s solution-fixed mouse backskin stained for Hsp105/HSP110 (green) using ab223620 at 1/100 dilution in immunohistochemical analysis. Secondary Antibody: Goat Anti-Rabbit FITC at 1/50 for 1 hour at RT.

  • Anti-Hsp105/HSP110 antibody (ab223620) at 1/1000 dilution + Human cell line lysates at 15 µg

    Secondary
    Donkey Anti-Rabbit IgG HRP

    Developed using the ECL technique.

    Predicted band size: 92,97 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 1.5% BSA.

    Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT. 

    Mix of human cell line lysates: epidermoid carcinoma (A431), adenocarcinomic alveolar basal epithelial cells (A549), colon cancer (HCT116), cervical cancer (HeLa), embryonic kidney (HEK293), liver cancer (HepG2), promyelocytic leukemia (HL-60), umbilical vein endothelial cells (HUVEC), T lymphocytes (Jurkat), breast cancer (MCF7), prostate cancer (PC3), brain glioblastom (T98G).

References

ab223620 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223620.
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