Key features and details
- Rabbit polyclonal to Hsp105/HSP110
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Hsp105/HSP110 antibody
See all Hsp105/HSP110 primary antibodies
DescriptionRabbit polyclonal to Hsp105/HSP110
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Hamster
Synthetic peptide corresponding to Hamster Hsp105/HSP110. Sequence identical to human and mouse.
Database link: Q60446
- IHC-P: Mouse backskin.
This product was previously labelled as Hsp105
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab223620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 92,97 kDa).|
FunctionPrevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
Tissue specificityHighly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
Sequence similaritiesBelongs to the heat shock protein 70 family.
modificationsPhosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
- Information by UniProt
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Bouin’s solution-fixed mouse backskin stained for Hsp105/HSP110 (green) using ab223620 at 1/100 dilution in immunohistochemical analysis. Secondary Antibody: Goat Anti-Rabbit FITC at 1/50 for 1 hour at RT.
Anti-Hsp105/HSP110 antibody (ab223620) at 1/1000 dilution + Human cell line lysates at 15 µg
Donkey Anti-Rabbit IgG HRP
Developed using the ECL technique.
Predicted band size: 92,97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Blocking buffer: 1.5% BSA.
Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.
Mix of human cell line lysates: epidermoid carcinoma (A431), adenocarcinomic alveolar basal epithelial cells (A549), colon cancer (HCT116), cervical cancer (HeLa), embryonic kidney (HEK293), liver cancer (HepG2), promyelocytic leukemia (HL-60), umbilical vein endothelial cells (HUVEC), T lymphocytes (Jurkat), breast cancer (MCF7), prostate cancer (PC3), brain glioblastom (T98G).
ab223620 has not yet been referenced specifically in any publications.