Overview

  • Product name

    Anti-Hsp105/HSP110 antibody [EPR4577]
    See all Hsp105/HSP110 primary antibodies
  • Description

    Rabbit monoclonal [EPR4577] to Hsp105/HSP110
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Hsp105/HSP110 aa 800-900. The exact sequence is proprietary.

  • Positive control

    • HeLa and HepG2 cell lysates; Human testis tissue; HeLa cells.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Hsp105

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4577
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 97 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
    • Tissue specificity

      Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
    • Sequence similarities

      Belongs to the heat shock protein 70 family.
    • Post-translational
      modifications

      Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Antigen NY CO 25 antibody
      • Antigen NY-CO-25 antibody
      • DKFZp686M05240 antibody
      • Heat shock 105kD alpha antibody
      • Heat shock 105kD antibody
      • Heat shock 105kD beta antibody
      • Heat shock 105kDa protein 1 antibody
      • Heat shock 105kDa protein antibody
      • Heat shock 105kDa/110kDa protein 1 antibody
      • Heat shock 110 kDa protein antibody
      • Heat shock 110kDa protein antibody
      • Heat shock protein 105 kDa antibody
      • HS105_HUMAN antibody
      • HSP105 antibody
      • HSP105A antibody
      • HSP105B antibody
      • HSP110 antibody
      • HSPH 1 antibody
      • Hsph1 antibody
      • KIAA0201 antibody
      • NY CO 25 antibody
      see all

    Images

    • All lanes : Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 97 kDa

    • ab108625 at 1/100 dilution staining Hsp105/HSP110 in Human testis by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab108625 at 1/100 dilution staining Hsp105/HSP110 in HeLa cells by Immunofluorescence.

    References

    This product has been referenced in:

    • Shen Y  et al. Tumor-derived exosomes educate dendritic cells to promote tumor metastasis via HSP72/HSP105-TLR2/TLR4 pathway. Oncoimmunology 6:e1362527 (2017). Read more (PubMed: 29209566) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab108625.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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