Recombinant Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free
See all Hsp105/HSP110 primary antibodies -
Description
Rabbit monoclonal [EPR4577] to Hsp105/HSP110 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Hsp105/HSP110 aa 800-900. The exact sequence is proprietary.
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General notes
ab247705 is the carrier-free version of ab108625 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247705 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Hsp105
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR4577 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab247705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 97 kDa. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended.
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Target
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Function
Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities. -
Tissue specificity
Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells. -
Sequence similarities
Belongs to the heat shock protein 70 family. -
Post-translational
modificationsPhosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10808 Human
- Omim: 610703 Human
- SwissProt: Q92598 Human
- Unigene: 36927 Human
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Alternative names
- Antigen NY CO 25 antibody
- Antigen NY-CO-25 antibody
- DKFZp686M05240 antibody
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Datasheets and documents
References
ab247705 has not yet been referenced specifically in any publications.
