RecombinantRabMAb

Recombinant Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)

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Western blot - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)
  • Flow Cytometry - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)
  • Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)
  • Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4577] to Hsp105/HSP110 - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free
    See all Hsp105/HSP110 primary antibodies

  • Description

    Rabbit monoclonal [EPR4577] to Hsp105/HSP110 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WB, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Hsp105/HSP110 aa 800-900. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, HepG2, and Caco-2 whole cell lysate. Human lung tissue lysate; IHC-P: Human testis tissue; ICC/IF: HeLa and HepG2 cells; Flow Cyt: HepG2 cells; IP: HeLa whole cell lysate.

  • General notes

    ab247705 is the carrier-free version of ab108625 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247705 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Hsp105

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4577

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab247705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 97 kDa.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.

    • Tissue specificity

      Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.

    • Sequence similarities

      Belongs to the heat shock protein 70 family.

    • Post-translational
      modifications

      Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • Antigen NY CO 25 antibody
        • Antigen NY-CO-25 antibody
        • DKFZp686M05240 antibody
        • Heat shock 105kD alpha antibody
        • Heat shock 105kD antibody
        • Heat shock 105kD beta antibody
        • Heat shock 105kDa protein 1 antibody
        • Heat shock 105kDa protein antibody
        • Heat shock 105kDa/110kDa protein 1 antibody
        • Heat shock 110 kDa protein antibody
        • Heat shock 110kDa protein antibody
        • Heat shock protein 105 kDa antibody
        • HS105_HUMAN antibody
        • HSP105 antibody
        • HSP105A antibody
        • HSP105B antibody
        • HSP110 antibody
        • HSPH 1 antibody
        • Hsph1 antibody
        • KIAA0201 antibody
        • NY CO 25 antibody
        see all

      Images

      • Western blot - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)
        Western blot - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)
        All lanes : Anti-Hsp105/HSP110 antibody [EPR4577] (ab108625) at 1/1000 dilution (Purified)

        Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
        Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
        Lane 3 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
        Lane 4 : Human lung lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 97 kDa
        Observed band size: 105 kDa
        why is the actual band size different from the predicted?



        Blocking/Diluting buffer: 5% NFDM/TBST

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108625).

      • Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)
        Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)

        Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Hsp105/HSP110 with Purified ab108625 at 1:50 dilution (2.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108625).

      • Flow Cytometry - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)
        Flow Cytometry - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)

        Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Hsp105/HSP110 with Purified ab108625 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108625).

      • Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)
        Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)

        Purified ab108625 at 1:20 dilution (0.6μg) immunoprecipitating Hsp105/HSP110 in HeLa whole cell lysate.
        Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
        Lane 2 (+): ab108625 + HeLa whole cell lysate.
        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108625 in HeLa whole cell lysate.
        VeriBlot for IP Detection Reagent (HRP) (ab131366) (1:1000 dilution) was used for Western blotting.
        Blocking Buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM/TBST.
        Observed band size: 105 kDa

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108625).

      • Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)
        Anti-Hsp105/HSP110 antibody [EPR4577] - BSA and Azide free (ab247705)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab247705? Please let us know so that we can cite the reference in this datasheet.

      ab247705 has not yet been referenced specifically in any publications.

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