Key features and details
- Rabbit polyclonal to HSP17.6
- Suitable for: WB
- Reacts with: Arabidopsis thaliana
- Isotype: IgG
Product nameAnti-HSP17.6 antibody
DescriptionRabbit polyclonal to HSP17.6
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Arabidopsis thaliana
Full length recombinant HSP17.6 protein produced in E. coli and purified by conventional methods (no affinity tag)
- Heat shocked (38° C for 2 hours) Arabidopsis thaliana lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab80183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
RelevanceHSP17.6 belongs to the class I family of small heat shock proteins. Small Hsps are induced when plant cells are stressed by increased temperature and appear to be involved in chaperone functions by protecting other proteins from irreversible denaturation. They are produced late in the seed maturation process.
- 17.6 kDa class I heat shock protein antibody
- Arabidopsis thaliana heat shock protein 17.6A antibody
- AT HSP17.6A antibody
Lanes 1-2 & 4-7 : Anti-HSP17.6 antibody (ab80183) at 1/1000 dilution
Lane 1 : Total protein from heat shocked Arabidopsis thaliana at 15 µg
Lane 2 : Arabidopsis thaliana control plant
Lane 3 : Empty lane
Lane 4 : Recombinant purified HSP17.6, 1 ng
Lane 5 : Recombinant purified HSP17.6, 2 ng
Lane 6 : Lysates from Arabidopsis thaliana, 5 ng
Lane 7 : recombinant purified HSP17.6, 10 ng
Lanes 1-2 & 4-7 : HRP-conjugated goat anti rabbit IgG
Predicted band size: 18 kDa
15% SDS-PAGE and blotted 1hour onto nitrocellulose
ab80183 has been referenced in 2 publications.
- Davies M et al. Stress in native grasses under ecologically relevant heat waves. PLoS One 13:e0204906 (2018). PubMed: 30308074
- Weng M et al. Histone chaperone ASF1 is involved in gene transcription activation in response to heat stress in Arabidopsis thaliana. Plant Cell Environ 37:2128-38 (2014). PubMed: 24548003