The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
HSP17.6 belongs to the class I family of small heat shock proteins. Small Hsps are induced when plant cells are stressed by increased temperature and appear to be involved in chaperone functions by protecting other proteins from irreversible denaturation. They are produced late in the seed maturation process.
Lane 1 : Total protein from heat shocked Arabidopsis thaliana at 15 µg Lane 2 : Arabidopsis thaliana control plant Lane 3 : Empty lane Lane 4 : Recombinant purified HSP17.6, 1 ng Lane 5 : Recombinant purified HSP17.6, 2 ng Lane 6 : Lysates from Arabidopsis thaliana, 5 ng Lane 7 : recombinant purified HSP17.6, 10 ng