Anti-Hsp20 antibody (ab13491)
Key features and details
- Rabbit polyclonal to Hsp20
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Hsp20 antibody
See all Hsp20 primary antibodies -
Description
Rabbit polyclonal to Hsp20 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Rat skeletal muscle Hsp20.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.268% PBS, 3% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 0.05 µg/ml. Detects a band of approximately 20 kDa.
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 0.05 µg/ml. Detects a band of approximately 20 kDa. |
Target
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Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock. - Information by UniProt
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Database links
- Entrez Gene: 126393 Human
- Entrez Gene: 192245 Rat
- Omim: 610695 Human
- SwissProt: O14558 Human
- SwissProt: P97541 Rat
- Unigene: 534538 Human
- Unigene: 3201 Rat
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Alternative names
- epididymis luminal protein 55 antibody
- FLJ32389 antibody
- Heat shock 20 kDa like protein p20 antibody
see all
Images
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Anti-Hsp20 antibody (ab13491) at 1/2000 dilution + Lysates prepared from rat heart tissue
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ICC/IF image of ab13491 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13491, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab13491 has been referenced in 8 publications.
- Lee B et al. Associations of Apoptotic and Anti-Apoptotic Factors with Beef Quality, Histochemical Characteristics, and Palatability of Hanwoo Longissimus thoracis Muscle. Animals (Basel) 12:N/A (2022). PubMed: 35203175
- Beuzelin D et al. Oral Delivery of miRNA With Lipidic Aminoglycoside Derivatives in the Breastfed Rat. Front Physiol 10:1037 (2019). PubMed: 31456698
- Hedegaard ER et al. Mechanisms involved in increased sensitivity to adenosine A(2A) receptor activation and hypoxia-induced vasodilatation in porcine coronary arteries. Eur J Pharmacol 723:216-26 (2014). WB . PubMed: 24309216
- Kirui DK et al. Transient mild hyperthermia induces E-selectin mediated localization of mesoporous silicon vectors in solid tumors. PLoS One 9:e86489 (2014). WB ; Human . PubMed: 24558362
- Chen A et al. Lysine deacetylase inhibition promotes relaxation of arterial tone and C-terminal acetylation of HSPB6 (Hsp20) in vascular smooth muscle cells. Physiol Rep 1:e00127 (2013). PubMed: 24400135
- Dordea AC et al. Differential vasodilation of human placental and myometrial arteries related to myofilament Ca2+-desensitization and the expression of Hsp20 but not MYPT1. Mol Hum Reprod N/A:N/A (2013). Human . PubMed: 23775458
- Zhou YC et al. Effects of buyang huanwu decoction on ventricular remodeling and differential protein profile in a rat model of myocardial infarction. Evid Based Complement Alternat Med 2012:385247 (2012). IHC-P ; Rat . PubMed: 23049607
- Fontaine JM et al. Interactions of HSP22 (HSPB8) with HSP20, alphaB-crystallin, and HSPB3. Biochem Biophys Res Commun 337:1006-11 (2005). WB ; African green monkey . PubMed: 16225851