Product nameAnti-Hsp20 antibody
See all Hsp20 primary antibodies
DescriptionRabbit polyclonal to Hsp20
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Cat, Dog, Human, Pig
Rat skeletal muscle Hsp20.
- HeLa cell lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.09% Sodium azide
Constituents: 0.268% PBS, 3% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab13491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 0.05 µg/ml. Detects a band of approximately 20 kDa.|
|IP||Use a concentration of 12.5 µg/ml.|
|IHC-P||Use at an assay dependent concentration. PubMed: 23049607|
Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
modificationsThe N-terminus is blocked.
Cellular localizationCytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
- Information by UniProt
- epididymis luminal protein 55 antibody
- FLJ32389 antibody
- Heat shock 20 kDa like protein p20 antibody
Anti-Hsp20 antibody (ab13491) at 1/2000 dilution + Lysates prepared from rat heart tissue
ICC/IF image of ab13491 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13491, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of rat ventricle tissue, staining Hsp20 with ab13491.
This product has been referenced in:
- Hedegaard ER et al. Mechanisms involved in increased sensitivity to adenosine A(2A) receptor activation and hypoxia-induced vasodilatation in porcine coronary arteries. Eur J Pharmacol 723:216-26 (2014). WB . Read more (PubMed: 24309216) »
- Kirui DK et al. Transient mild hyperthermia induces E-selectin mediated localization of mesoporous silicon vectors in solid tumors. PLoS One 9:e86489 (2014). WB ; Human . Read more (PubMed: 24558362) »