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    hsp20-antibody-epr14457-n-terminal-ab188864.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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RecombinantRabMAb

Recombinant Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
  • Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
  • Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14457] to Hsp20 - N-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Hsp20 antibody [EPR14457] - N-terminal
    See all Hsp20 primary antibodies
  • Description

    Rabbit monoclonal [EPR14457] to Hsp20 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skeletal muscle and rat heart lysates; Human transitional cell carcinoma of bladder tissue; HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14457
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Heart
    • Apoptosis

Associated products

  • Alternative Versions

    • Anti-Hsp20 antibody [EPR14457] - BSA and Azide free (ab250977)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Recombinant Human Hsp20 protein (ab52077)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.

Target

  • Sequence similarities

    Belongs to the small heat shock protein (HSP20) family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
  • Target information above from: UniProt accession O14558 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 126393 Human
    • Entrez Gene: 243912 Mouse
    • Entrez Gene: 192245 Rat
    • Omim: 610695 Human
    • SwissProt: O14558 Human
    • SwissProt: Q5EBG6 Mouse
    • SwissProt: P97541 Rat
    • Unigene: 534538 Human
    • Unigene: 34885 Mouse
    • Unigene: 3201 Rat
    see all
  • Alternative names

    • epididymis luminal protein 55 antibody
    • FLJ32389 antibody
    • Heat shock 20 kDa like protein p20 antibody
    • Heat shock 20 kDa-like protein p20 antibody
    • Heat shock protein alpha crystallin related B6 antibody
    • Heat shock protein beta 6 antibody
    • Heat shock protein beta-6 antibody
    • Heat shock protein, 20-KD antibody
    • Heat-shock 27-KD protein 6 antibody
    • HEL55 antibody
    • Hsp20 antibody
    • HspB6 antibody
    • HSPB6_HUMAN antibody
    see all

Images

  • Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864) at 1/1000 dilution + Rat heart lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/500 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling Hsp20 with ab188864 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Hsp20 with ab188864 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab188864? Please let us know so that we can cite the reference in this datasheet.

ab188864 has not yet been referenced specifically in any publications.

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