Recombinant
RabMAb

Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

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Overview

  • Product name
    Anti-Hsp20 antibody [EPR14457] - N-terminal
    See all Hsp20 primary antibodies
  • Description
    Rabbit monoclonal [EPR14457] to Hsp20 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Hsp20 aa 1-100. The exact sequence is proprietary.
    Database link: O14558

  • Positive control
    • Human skeletal muscle and rat heart lysates; Human transitional cell carcinoma of bladder tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/50 - 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.

Target

Images

  • Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864) at 1/1000 dilution + Rat heart lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/500 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling Hsp20 with ab188864 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Hsp20 with ab188864 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

References

ab188864 has not yet been referenced specifically in any publications.

Publishing research using ab188864? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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