RecombinantRabMAb

Recombinant Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

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Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
  • Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
  • Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14457] to Hsp20 - N-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Hsp20 antibody [EPR14457] - N-terminal
    See all Hsp20 primary antibodies

  • Description

    Rabbit monoclonal [EPR14457] to Hsp20 - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skeletal muscle and rat heart lysates; Human transitional cell carcinoma of bladder tissue; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.

Target

Images

  • Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Western blot - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864) at 1/1000 dilution + Rat heart lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/500 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling Hsp20 with ab188864 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Hsp20 with ab188864 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)
    Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864)

References (0)

Publishing research using ab188864? Please let us know so that we can cite the reference in this datasheet.

ab188864 has not yet been referenced specifically in any publications.

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