Recombinant Anti-Hsp20 antibody [EPR14458] (ab184161)
![Western blot - Anti-Hsp20 antibody [EPR14458] (ab184161)](https://www.abcam.com/ps/products/184/ab184161/Images/ab184161-216438-anti-hsp20-antibody-epr14458-western-blot.jpg "Western blot - Anti-Hsp20 antibody [EPR14458] (ab184161)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14458] to Hsp20
- Suitable for: IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Hsp20 antibody [EPR14458]
See all Hsp20 primary antibodies -
Description
Rabbit monoclonal [EPR14458] to Hsp20 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle lysate; Human and mouse skeletal muscle tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14458 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| WB |
1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
|
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| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock. - Information by UniProt
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Database links
- Entrez Gene: 126393 Human
- Entrez Gene: 243912 Mouse
- Entrez Gene: 192245 Rat
- Omim: 610695 Human
- SwissProt: O14558 Human
- SwissProt: Q5EBG6 Mouse
- SwissProt: P97541 Rat
- Unigene: 534538 Human
see all -
Alternative names
- epididymis luminal protein 55 antibody
- FLJ32389 antibody
- Heat shock 20 kDa like protein p20 antibody
see all
Images
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Western blot - Anti-Hsp20 antibody [EPR14458] (ab184161)Anti-Hsp20 antibody [EPR14458] (ab184161) at 1/50000 dilution + Human skeletal muscle lysate at 20 µg
Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14458] (ab184161)](https://www.abcam.com/ps/products/184/ab184161/Images/ab184161-216439-anti-hsp20-antibody-epr14458-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14458] (ab184161)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Hsp20 with ab184161 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14458] (ab184161)](https://www.abcam.com/ps/products/184/ab184161/Images/ab184161-216440-anti-hsp20-antibody-epr14458-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 antibody [EPR14458] (ab184161)Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Hsp20 with ab184161 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-Hsp20 antibody [EPR14458] (ab184161)](https://www.abcam.com/ps/products/184/ab184161/Images/ab184161-216441-anti-hsp20-antibody-epr14458-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Hsp20 antibody [EPR14458] (ab184161)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Hsp20 with ab184161 at 1/100 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green) followed by Goat anti-rabbit IgG (FITC) secondary antibodyat 1/150 dilution.
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![Anti-Hsp20 antibody [EPR14458] (ab184161)](https://www.abcam.com/ps/products/184/ab184161/Images/ab184161-5-benefits-of-recombinant-antibodies.png)
Anti-Hsp20 antibody [EPR14458] (ab184161)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (7)
ab184161 has been referenced in 7 publications.
- Chen YF et al. MicroRNA-23a-5p Is Involved in the Regulation of Lipopolysaccharide-Induced Acute Lung Injury by Targeting HSP20/ASK1. Oxid Med Cell Longev 2021:9942557 (2021). PubMed: 34422215
- Bao A et al. Inducible expression of heat shock protein 20 protects airway epithelial cells against oxidative injury involving the Nrf2-NQO-1 pathway. Cell Biosci 10:120 (2020). PubMed: 33088476
- Silva KAS et al. Tissue-specific small heat shock protein 20 activation is not associated with traditional autophagy markers in Ossabaw swine with cardiometabolic heart failure. Am J Physiol Heart Circ Physiol 319:H1036-H1043 (2020). PubMed: 32946285
- Chen X et al. Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Front Oncol 9:523 (2019). PubMed: 31293967
- Gorter RP et al. Heat shock proteins are differentially expressed in brain and spinal cord: implications for multiple sclerosis. Clin Exp Immunol N/A:N/A (2018). PubMed: 30014472
- Lai PF et al. Differential impact of acute and prolonged cAMP agonist exposure on protein kinase A activation and human myometrium contractile activity. J Physiol 594:6369-6393 (2016). PubMed: 27328735
- Zhong B et al. Hsp20 Protects against Oxygen-Glucose Deprivation/Reperfusion-Induced Golgi Fragmentation and Apoptosis through Fas/FasL Pathway. Oxid Med Cell Longev 2015:606934 (2015). IP . PubMed: 26199678
