Anti-Hsp22/HSPB8 antibody (ab79784)
Key features and details
- Rabbit polyclonal to Hsp22/HSPB8
- Suitable for: IHC-P, WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Hsp22/HSPB8 antibody
See all Hsp22/HSPB8 primary antibodies -
Description
Rabbit polyclonal to Hsp22/HSPB8 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length native protein (purified) corresponding to Human Hsp22/HSPB8.
Database link: Q9UJY1 -
Positive control
- WB: Rat skeletal tissue. IHC-P: Mouse spinal cord and epithelial tissue. ICC: Human HaCaT cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100.
|
|
WB |
1/1000. Predicted molecular weight: 22 kDa.
|
|
ICC |
1/100.
|
Notes |
---|
IHC-P
1/100. |
WB
1/1000. Predicted molecular weight: 22 kDa. |
ICC
1/100. |
Target
-
Function
Displays temperature-dependent chaperone activity. -
Tissue specificity
Predominantly expressed in skeletal muscle and heart. -
Involvement in disease
Neuronopathy, distal hereditary motor, 2A
Charcot-Marie-Tooth disease 2L -
Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Cellular localization
Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock. - Information by UniProt
-
Database links
- Entrez Gene: 26353 Human
- Entrez Gene: 80888 Mouse
- Entrez Gene: 113906 Rat
- Omim: 608014 Human
- SwissProt: Q9UJY1 Human
- SwissProt: Q9JK92 Mouse
- SwissProt: Q9EPX0 Rat
- Unigene: 400095 Human
see all -
Alternative names
- Alpha crystallin C chain antibody
- Alpha-crystallin C chain antibody
- Charcot Marie Tooth disease axonal type 2L antibody
see all
Images
-
Anti-Hsp22/HSPB8 antibody (ab79784) at 20 µg + Rat skeletal muscle lysate
Predicted band size: 22 kDa -
ab79784 at 1/100 dilution staining by Hsp22/HSPB8 in mouse spinal cord tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/10 dilution.
-
Immunohistochemical analysis of Bouin's fixed paraffin-embedded mouse epithelial tissue using ab79784 at 1/100 dilution for 1 hour at RT, followed by secondary Goat anti-rabbit (green) at 1/50 diltuon for 1 hour at RT.
-
Immunocytochemical analysis of human HaCaT cells fixed with 100% cold methanol at -20°C for 10 minutes using ab79784 at 1/100 dilution for 12 hours at 4°C, followed by secondary Goat anti-rabbit (green) at 1/50 dilution for 1-2 hours at RT in the dark.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab79784 has been referenced in 4 publications.
- Johari M et al. Missense mutations in small muscle protein X-linked (SMPX) cause distal myopathy with protein inclusions. Acta Neuropathol 142:375-393 (2021). PubMed: 33974137
- Yao J et al. The Homeobox gene, HOXB13, Regulates a Mitotic Protein-Kinase Interaction Network in Metastatic Prostate Cancers. Sci Rep 9:9715 (2019). PubMed: 31273254
- Bouhy D et al. A knock-in/knock-out mouse model of HSPB8-associated distal hereditary motor neuropathy and myopathy reveals toxic gain-of-function of mutant Hspb8. Acta Neuropathol 135:131-148 (2018). IHC . PubMed: 28780615
- Ghaoui R et al. Mutations in HSPB8 causing a new phenotype of distal myopathy and motor neuropathy. Neurology 86:391-8 (2016). PubMed: 26718575