Key features and details
- Rabbit polyclonal to Hsp22/HSPB8
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Hsp22/HSPB8 antibody
See all Hsp22/HSPB8 primary antibodies
DescriptionRabbit polyclonal to Hsp22/HSPB8
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Hsp22/HSPB8 aa 134-196. The exact sequence is proprietary.
Database link: Q9UJY1
- WB: HEK-293T, A431 cell lysate. ICC/IF: HeLa cells. IHC-P: Human lung SCC. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skeletal muscle.
This product was previously labelled as Hsp22
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/3000. Predicted molecular weight: 22 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionDisplays temperature-dependent chaperone activity.
Tissue specificityPredominantly expressed in skeletal muscle and heart.
Involvement in diseaseNeuronopathy, distal hereditary motor, 2A
Charcot-Marie-Tooth disease 2L
Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
Cellular localizationCytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
- Information by UniProt
- Alpha crystallin C chain antibody
- Alpha-crystallin C chain antibody
- Charcot Marie Tooth disease axonal type 2L antibody
All lanes : Anti-Hsp22/HSPB8 antibody (ab96837) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
12% SDS PAGE
Paraffin embedded human lung SCC tissue stained for HSP22 using ab96837 at 1/500 dilution in immunohistochemical analysis.
Immunofluorescence analysis of Hsp22/HSPB8 expression in PFA-fixed HeLa cells, using ab96837 at 1/200 dilution. Lower image was merged with DNA probe.
IHC image of Hsp22/HSPB8 staining in Human normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96837, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab96837 has been referenced in 3 publications.
- Gorter RP et al. Heat shock proteins are differentially expressed in brain and spinal cord: implications for multiple sclerosis. Clin Exp Immunol N/A:N/A (2018). PubMed: 30014472
- Shen J et al. HSPB8 promotes cancer cell growth by activating the ERK-CREB pathway and is indicative of a poor prognosis in gastric cancer patients. Oncol Rep 39:2978-2986 (2018). PubMed: 29693129
- Judge LM et al. A BAG3 chaperone complex maintains cardiomyocyte function during proteotoxic stress. JCI Insight 2:N/A (2017). PubMed: 28724793