Overview

  • Product name

  • Description

    Rabbit polyclonal to Hsp22/HSPB8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Hsp22/HSPB8 aa 134-196.

  • Positive control

    • WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate. ICC/IF: HeLa cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skeletal muscle.
  • General notes

     This product was previously labelled as Hsp22

     

Properties

Applications

Our Abpromise guarantee covers the use of ab96837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 22 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Displays temperature-dependent chaperone activity.
  • Tissue specificity

    Predominantly expressed in skeletal muscle and heart.
  • Involvement in disease

    Neuronopathy, distal hereditary motor, 2A
    Charcot-Marie-Tooth disease 2L
  • Sequence similarities

    Belongs to the small heat shock protein (HSP20) family.
  • Cellular localization

    Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha crystallin C chain antibody
    • Alpha-crystallin C chain antibody
    • Charcot Marie Tooth disease axonal type 2L antibody
    • Charcot Marie Tooth disease spinal antibody
    • CMT2L antibody
    • CRYAC antibody
    • DHMN 2 antibody
    • DHMN2 antibody
    • E2 induced gene 1 protein antibody
    • E2-induced gene 1 protein antibody
    • E2IG1 antibody
    • H11 antibody
    • Heat shock 22kDa protein 8 antibody
    • Heat shock 27kDa protein 8 antibody
    • Heat shock protein 22 antibody
    • Heat shock protein beta 8 antibody
    • Heat shock protein beta-8 antibody
    • Hereditary motor neuropathy distal antibody
    • HMN 2 antibody
    • HMN2 antibody
    • HMN2A antibody
    • HSB8 antibody
    • HSPB 8 antibody
    • HspB8 antibody
    • HSPB8_HUMAN antibody
    • OTTHUMP00000239768 antibody
    • Protein kinase H11 antibody
    • Small stress protein like protein HSP22 antibody
    • Small stress protein-like protein HSP22 antibody
    • Spinal muscular atrophy distal adult autosomal dominant antibody
    see all

Images

  • All lanes : Anti-Hsp22/HSPB8 antibody (ab96837) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 22 kDa



    12% SDS PAGE
  • IHC image of Hsp22/HSPB8 staining in Human normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96837, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunofluorescence analysis of Hsp22/HSPB8 expression in PFA-fixed HeLa cells, using ab96837 at 1/200 dilution. Lower image was merged with DNA probe.

References

This product has been referenced in:

  • Gorter RP  et al. Heat shock proteins are differentially expressed in brain and spinal cord: implications for multiple sclerosis. Clin Exp Immunol N/A:N/A (2018). Read more (PubMed: 30014472) »
  • Judge LM  et al. A BAG3 chaperone complex maintains cardiomyocyte function during proteotoxic stress. JCI Insight 2:N/A (2017). Read more (PubMed: 28724793) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
IHC - Wholemount
Sample
Zebrafish Embryo (muscle tissue)
Specification
muscle tissue

Abcam user community

Verified customer

Submitted Oct 07 2013

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