Recombinant Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9714] to Hsp22/HSPB8
- Suitable for: ICC/IF, IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Hsp22/HSPB8 antibody [EPR9714]
See all Hsp22/HSPB8 primary antibodies -
Description
Rabbit monoclonal [EPR9714] to Hsp22/HSPB8 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Hsp22/HSPB8. The immunogen used for this product shares 64% homology with Hsp27. Cross-reactivity with this protein has not been confirmed experimentally.
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Positive control
- Fetal heart and fetal muscle lysates; HeLa cell lysate; Human skeletal muscle tissue; A673 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9714 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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Notes |
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ICC/IF
1/50 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
Target
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Function
Displays temperature-dependent chaperone activity. -
Tissue specificity
Predominantly expressed in skeletal muscle and heart. -
Involvement in disease
Neuronopathy, distal hereditary motor, 2A
Charcot-Marie-Tooth disease 2L -
Sequence similarities
Belongs to the small heat shock protein (HSP20) family. -
Cellular localization
Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock. - Information by UniProt
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Database links
- Entrez Gene: 26353 Human
- Entrez Gene: 80888 Mouse
- Entrez Gene: 113906 Rat
- Omim: 608014 Human
- SwissProt: Q9UJY1 Human
- SwissProt: Q9JK92 Mouse
- SwissProt: Q9EPX0 Rat
- Unigene: 400095 Human
see all -
Alternative names
- Alpha crystallin C chain antibody
- Alpha-crystallin C chain antibody
- Charcot Marie Tooth disease axonal type 2L antibody
see all
Images
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All lanes : Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552) at 1/1000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HSPB8 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 21 kDaLanes 1 - 2: Merged signal (red and green). Green - ab151552 observed at 212 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab151552 was shown to react with Hsp22/HSPB8 in wild-type HeLa cells in Western blot with loss of signal observed in HSPB8 knockout cell line ab265112 (HSPB8 knockout cell lysate ab257468). Wild-type HeLa and HSPB8 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab151552 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Hsp22/HSPB8 with ab151552 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of A673 cells labeling Hsp22/HSPB8 with ab151552 at 1/50 dilution.
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All lanes : Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552) at 1/1000 dilution
Lane 1 : Fetal heart lysate
Lane 2 : Fetal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21 kDa -
Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab151552 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab151552 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Gastric carcinoma tissue using ab151552 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Mouse brain tissue using ab151552 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151552 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab151552 has been referenced in 7 publications.
- Yu Y et al. Hsp22 ameliorates lipopolysaccharide-induced myocardial injury by inhibiting inflammation, oxidative stress, and apoptosis. Bioengineered 12:12544-12554 (2021). PubMed: 34839787
- Chen Q et al. Atorvastatin downregulates HSP22 expression in an atherosclerotic model in vitro and in vivo. Int J Mol Med 43:821-829 (2019). PubMed: 30535427
- Yu L et al. HSP22 suppresses diabetes-induced endothelial injury by inhibiting mitochondrial reactive oxygen species formation. Redox Biol 21:101095 (2019). PubMed: 30640127
- Li F et al. Heat Shock Protein B8 (HSPB8) Reduces Oxygen-Glucose Deprivation/Reperfusion Injury via the Induction of Mitophagy. Cell Physiol Biochem 48:1492-1504 (2018). PubMed: 30071537
- Seminary ER et al. Modeling Protein Aggregation and the Heat Shock Response in ALS iPSC-Derived Motor Neurons. Front Neurosci 12:86 (2018). PubMed: 29515358
- Jiang B et al. MicroRNA-126a-5p enhances myocardial ischemia-reperfusion injury through suppressing Hspb8 expression. Oncotarget 8:94172-94187 (2017). PubMed: 29212219
- Unger A et al. Translocation of molecular chaperones to the titin springs is common in skeletal myopathy patients and affects sarcomere function. Acta Neuropathol Commun 5:72 (2017). PubMed: 28915917