Name: Anti-Hsp22/HSPB8 antibody [EPR9714]
Brand: RabMAb
SKU: ab151552
Rating: 5 (2 reviews)

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Western blot - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9714] to Hsp22/HSPB8
Suitable for: ICC/IF, IHC-P, WB
Knockout validated
Reacts with: Mouse, Human

Carrier Free

Product name

Anti-Hsp22/HSPB8 antibody [EPR9714]
See all Hsp22/HSPB8 primary antibodies
Description

Rabbit monoclonal [EPR9714] to Hsp22/HSPB8
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, IHC-P, WBmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Human
Predicted to work with: Rat
Immunogen

Synthetic peptide corresponding to Human Hsp22/HSPB8. The immunogen used for this product shares 64% homology with Hsp27. Cross-reactivity with this protein has not been confirmed experimentally.
Positive control
- Fetal heart and fetal muscle lysates; HeLa cell lysate; Human skeletal muscle tissue; A673 cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9714
Isotype

IgG
Research areas
- Neuroscience
- Processes

Alternative Versions
- Anti-Hsp22/HSPB8 antibody [EPR9714] - BSA and Azide free (ab248999)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human HSPB8 (Hsp22) knockout HeLa cell line (ab265112)
KO cell lysates
- Human HSPB8 (Hsp22) knockout HeLa cell lysate (ab257468)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/50 - 1/100.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB	(1)	1/1000 - 1/10000. Predicted molecular weight: 21 kDa.

Notes
ICC/IF 1/50 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.

Application notes

Is unsuitable for IP.

Function

Displays temperature-dependent chaperone activity.
Tissue specificity

Predominantly expressed in skeletal muscle and heart.
Involvement in disease

Neuronopathy, distal hereditary motor, 2A
Charcot-Marie-Tooth disease 2L
Sequence similarities

Belongs to the small heat shock protein (HSP20) family.
Cellular localization

Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
Target information above from: UniProt accession Q9UJY1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 26353 Human
- Entrez Gene: 80888 Mouse
- Entrez Gene: 113906 Rat
- Omim: 608014 Human
- SwissProt: Q9UJY1 Human
- SwissProt: Q9JK92 Mouse
- SwissProt: Q9EPX0 Rat
- Unigene: 400095 Human
- Unigene: 21549 Mouse
- Unigene: 102906 Rat
see all
Alternative names
- Alpha crystallin C chain antibody
- Alpha-crystallin C chain antibody
- Charcot Marie Tooth disease axonal type 2L antibody
- Charcot Marie Tooth disease spinal antibody
- CMT2L antibody
- CRYAC antibody
- DHMN 2 antibody
- DHMN2 antibody
- E2 induced gene 1 protein antibody
- E2-induced gene 1 protein antibody
- E2IG1 antibody
- H11 antibody
- Heat shock 22kDa protein 8 antibody
- Heat shock 27kDa protein 8 antibody
- Heat shock protein 22 antibody
- Heat shock protein beta 8 antibody
- Heat shock protein beta-8 antibody
- Hereditary motor neuropathy distal antibody
- HMN 2 antibody
- HMN2 antibody
- HMN2A antibody
- HSB8 antibody
- HSPB 8 antibody
- HspB8 antibody
- HSPB8_HUMAN antibody
- OTTHUMP00000239768 antibody
- Protein kinase H11 antibody
- Small stress protein like protein HSP22 antibody
- Small stress protein-like protein HSP22 antibody
- Spinal muscular atrophy distal adult autosomal dominant antibody
see all

Western blot - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

All lanes : Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552) at 1/1000 dilution

Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HSPB8 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 21 kDa
Observed band size: 212 kDa why is the actual band size different from the predicted?

Lanes 1 - 2: Merged signal (red and green). Green - ab151552 observed at 212 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab151552 was shown to react with Hsp22/HSPB8 in wild-type HeLa cells in Western blot with loss of signal observed in HSPB8 knockout cell line ab265112 (HSPB8 knockout cell lysate ab257468). Wild-type HeLa and HSPB8 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab151552 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Hsp22/HSPB8 with ab151552 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

Immunofluorescent analysis of A673 cells labeling Hsp22/HSPB8 with ab151552 at 1/50 dilution.
Western blot - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

All lanes : Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552) at 1/1000 dilution

Lane 1 : Fetal heart lysate
Lane 2 : Fetal muscle lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab151552 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab151552 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

Immunohistochemical analysis of paraffin embedded Human Gastric carcinoma tissue using ab151552 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

Immunohistochemical analysis of paraffin embedded Mouse brain tissue using ab151552 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151552 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab151552? Please let us know so that we can cite the reference in this datasheet.

ab151552 has been referenced in 6 publications.

Chen Q et al. Atorvastatin downregulates HSP22 expression in an atherosclerotic model in vitro and in vivo. Int J Mol Med 43:821-829 (2019). PubMed: 30535427
Yu L et al. HSP22 suppresses diabetes-induced endothelial injury by inhibiting mitochondrial reactive oxygen species formation. Redox Biol 21:101095 (2019). PubMed: 30640127
Li F et al. Heat Shock Protein B8 (HSPB8) Reduces Oxygen-Glucose Deprivation/Reperfusion Injury via the Induction of Mitophagy. Cell Physiol Biochem 48:1492-1504 (2018). PubMed: 30071537
Seminary ER et al. Modeling Protein Aggregation and the Heat Shock Response in ALS iPSC-Derived Motor Neurons. Front Neurosci 12:86 (2018). PubMed: 29515358
Jiang B et al. MicroRNA-126a-5p enhances myocardial ischemia-reperfusion injury through suppressing Hspb8 expression. Oncotarget 8:94172-94187 (2017). PubMed: 29212219
Unger A et al. Translocation of molecular chaperones to the titin springs is common in skeletal myopathy patients and affects sarcomere function. Acta Neuropathol Commun 5:72 (2017). PubMed: 28915917

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Heart)

Permeabilization

Yes - Tween-20

Specification

Heart

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 22 2016

Application

Western blot

Sample

Mouse Tissue lysate - whole (Heart)

Gel Running Conditions

Reduced Denaturing (4-12%)

Loading amount

20 µg

Specification

Heart

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 09 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

KO cell lines

KO cell lysates

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (6)

Customer reviews and Q&As

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Immunohistochemistry (Frozen sections) abreview for Anti-Hsp22/HSPB8 antibody [EPR9714]

Western blot abreview for Anti-Hsp22 antibody [EPR9714]