• Product name

  • Description

    Rabbit polyclonal to Hsp27
  • Host species

  • Specificity

    This antibody detects an 25 kDa protein, corresponding to the apparent molecular mass of Heat Shock Protein 27 (Hsp27) on SDS-PAGE immunoblots. This antibody has been shown to react with both the phosphorylated and the non-phosphorylated forms of Hsp27. Note: It has been reported that certain murine cell lines do not express Hsp27 under certain conditions. This antibody also recognizes a mitochondrial small Hsp (35 kDa) in rat PC12 cells. This mitochondrial small Hsp is proposed to protect mitochondrial complex I from oxidative stress.
  • Tested applications

    Suitable for: IHC, ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Pig, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Hsp27.
    Database link: P04792

  • Positive control

    • WB: HSP27 Recombinant Human Protein, HeLa and PC-12 cell lysates; Rat keratinocytes, whole cell lysate IHC: Human heart tissue
  • General notes

    For maximum product recovery, after thawing, centrifuge the product vial before removing cap.



Our Abpromise guarantee covers the use of ab12351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB 1/1 - 1/1000. Predicted molecular weight: 25 kDa.
IP 1/1 - 1/100.


  • Function

    Involved in stress resistance and actin organization.
  • Tissue specificity

    Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
  • Involvement in disease

    Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
    Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
  • Sequence similarities

    Belongs to the small heat shock protein (HSP20) family.
  • Post-translational

    Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • Database links

  • Alternative names

    • Heat shock 27kDa protein antibody
    • 28 kDa heat shock protein antibody
    • CMT2F antibody
    • DKFZp586P1322 antibody
    • epididymis secretory protein Li 102 antibody
    • Estrogen regulated 24 kDa protein antibody
    • Estrogen-regulated 24 kDa protein antibody
    • Heat shock 25kDa protein 1 antibody
    • Heat shock 27 kDa protein antibody
    • Heat shock 27kD protein 1 antibody
    • Heat shock 27kDa protein 1 antibody
    • Heat shock 28kDa protein 1 antibody
    • Heat Shock Protein 27 antibody
    • Heat shock protein beta 1 antibody
    • Heat shock protein beta-1 antibody
    • heat shock protein family B (small) member 1 antibody
    • HEL-S-102 antibody
    • HMN2B antibody
    • HS.76067 antibody
    • Hsp 25 antibody
    • HSP 27 antibody
    • Hsp 28 antibody
    • Hsp B1 antibody
    • Hsp25 antibody
    • HSP27 antibody
    • Hsp28 antibody
    • HspB1 antibody
    • HSPB1_HUMAN antibody
    • SRP27 antibody
    • Stress responsive protein 27 antibody
    • Stress-responsive protein 27 antibody
    see all


  • All lanes : Anti-Hsp27 antibody (ab12351) at 1 µg/ml

    Lane 1 : MW Marker
    Lane 2 : HSP27 Recombinant Human Protein
    Lane 3 : HSP25 Recombinant Murine Protein (Negative Control)
    Lane 4 : HeLa cell lysate
    Lane 5 : HeLa cell lysate, Heat Shocked
    Lane 6 : Vero, Heat Shocked
    Lane 7 : 3T3 cell lysate, heat shocked
    Lane 8 : PC-12 cell lysate, Heat Shocked

    Predicted band size: 25 kDa

  • Immunohistochemical analysis of human heart tissue sections with ab12351 at 10 µg/mL.

  • Anti-Hsp27 antibody (ab12351) at 1/500 dilution + Rat keratinocytes, whole cell lysate at 10 µg

    HRP conjugated goat anti-rabbit antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa
    Additional bands at: 120 kDa (possible non-specific binding), 215 kDa (possible non-specific binding)

    Exposure time: 5 minutes

    See Abreview

  • All lanes : Anti-Hsp27 antibody (ab12351) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from untreated PC-12 cells
    Lane 3 : Cell lysates prepared from heat shock treated PC-12 cells

    Predicted band size: 25 kDa


This product has been referenced in:

See all 8 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Western blot
Mouse Tissue lysate - whole (mouse colon, mouse heart lysate made in 1%SDS)
Gel Running Conditions
Reduced Denaturing (4-12% NuPage Bis-tris)
Loading amount
30 µg
Tissues isolated in 1% SDS with homogenization. No protease- or phosphatase-inhibitors added. Proteins not degraded as evaluated by other immunoblots with this tissue.
mouse colon, mouse heart lysate made in 1%SDS
Blocking step
Unknown - proprietary iBlot system for fluoresence detection. as blocking agent for 5 minute(s) · Concentration: .1% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 26 2019

Western blot
Rat Cell lysate - whole cell (Keratinocytes)
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 26 2008

Immunocytochemistry/ Immunofluorescence
Rat Cell (Keratinocyte)
Yes - Triton X
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.8% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 25 2008

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