Key features and details
- Rabbit polyclonal to Hsp27
- Suitable for: WB, ICC/IF
- Reacts with: Cow, Human
- Isotype: IgG
Product nameAnti-Hsp27 antibody
See all Hsp27 primary antibodies
DescriptionRabbit polyclonal to Hsp27
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Cow, Human
Recombinant full length protein (His-tag) corresponding to Cow Hsp27 aa 1-201. (Expressed in E.coli).
MAERRVPFSLLRGPSWDPFRDWYPAHSRLFDQAFGLPRLPEEWSQWLSHS GWPGYVRALPAAAIEGPAYNRALSRQLSSGVSEIQQTADRWRVSLDVNHF APEELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTLVS SSLSPEGTLTVEAPLPKSATQSAEITIPVTFQARAQLGGPEAGKSEQPEN K
Database link: Q3T149
- ICC/IF: HeLa cells. WB: Cow serum; Cow heart and skeletal muscle lysates; HeLa cell lysate; Recombinant cow Hsp27 protein.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231552 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 22 kDa.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionInvolved in stress resistance and actin organization.
Tissue specificityDetected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Involvement in diseaseDefects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
modificationsPhosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
- Information by UniProt
- Heat shock 27kDa protein antibody
- 28 kDa heat shock protein antibody
- CMT2F antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp27 (green) using ab231552 at 20 µg/ml in ICC/IF, followed by an FITC conjugated secondary antibody.
Anti-Hsp27 antibody (ab231552) at 3 µg/ml + Recombinant cow Hsp27 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 22 kDa
All lanes : Anti-Hsp27 antibody (ab231552) at 3 µg/ml
Lane 1 : Cow serum
Lane 2 : Cow heart lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : Cow skeletal muscle lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 22 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231552 has not yet been referenced specifically in any publications.