• Product name
    Anti-Hsp27 antibody [B317]
    See all Hsp27 primary antibodies
  • Description
    Mouse monoclonal [B317] to Hsp27
  • Host species
  • Specificity
    This antibody, also known as the estrogen regulated 24 kD protein and HSP 28, is one of the several heat shock proteins (HSP) produced by all organisms studied. The antigen is induced by elevated temperature as well as estrogen in hormone response. This protein has been studied in human estrogen response tissues such as cervix, endometrium and breast tissue. It may be useful in classification of various hormone sensitive tumors.
  • Tested applications
    Suitable for: IHC-FoFr, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Hsp27. Partially purified HSP27 derived from MCF-7 cytosol.

  • Positive control
    • Breast Carcinoma.
  • General notes
    Prolonged fixation in buffered formalin can destroy the epitope.



Our Abpromise guarantee covers the use of ab8600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/50 - 1/100.
IHC-Fr 1/50 - 1/100.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/300.


  • Function
    Involved in stress resistance and actin organization.
  • Tissue specificity
    Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
  • Involvement in disease
    Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
    Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
  • Sequence similarities
    Belongs to the small heat shock protein (HSP20) family.
  • Post-translational
    Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heat shock 27kDa protein antibody
    • 28 kDa heat shock protein antibody
    • CMT2F antibody
    • DKFZp586P1322 antibody
    • epididymis secretory protein Li 102 antibody
    • Estrogen regulated 24 kDa protein antibody
    • Estrogen-regulated 24 kDa protein antibody
    • Heat shock 25kDa protein 1 antibody
    • Heat shock 27 kDa protein antibody
    • Heat shock 27kD protein 1 antibody
    • Heat shock 27kDa protein 1 antibody
    • Heat shock 28kDa protein 1 antibody
    • Heat Shock Protein 27 antibody
    • Heat shock protein beta 1 antibody
    • Heat shock protein beta-1 antibody
    • heat shock protein family B (small) member 1 antibody
    • HEL-S-102 antibody
    • HMN2B antibody
    • HS.76067 antibody
    • Hsp 25 antibody
    • HSP 27 antibody
    • Hsp 28 antibody
    • Hsp B1 antibody
    • Hsp25 antibody
    • HSP27 antibody
    • Hsp28 antibody
    • HspB1 antibody
    • HSPB1_HUMAN antibody
    • SRP27 antibody
    • Stress responsive protein 27 antibody
    • Stress-responsive protein 27 antibody
    see all


  • All lanes : Anti-Hsp27 antibody [B317] (ab8600) at 1/300 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size: 23 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 26 kDa (possible post-translational modification)

  • ab8600 staining Hsp27 in human normal skin tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation and heat mediated antigen retrieval in Citrate buffer pH 6.0. The primary antibody was diluted, 1/50 in PBT (PBS + 0.5% Tween-20 + 0.5% BSA) and incubated with sample for 20 hours at 21°C. An Alexa Fluor® 594 conjugated Donkey polyclonal to mouse IgG
    was used as secondary at 1/100 dilution, for 1 hour at 21°C. ab8600 show red staining of Hsp27 in image. Counterstain with DAPI was performed by adding DAPI in a final concentration of 0.5 µg/ml to the mounting medium.

    See Abreview

  • All lanes : Anti-Hsp27 antibody [B317] (ab8600) at 0.83 µg/ml

    All lanes : Human HaCaT Whole cell lysate

    Lysates/proteins at 30 µg per lane.

    All lanes : An HRP-conjugated goat anti-mouse polyclonal at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 5 minutes

    Blocking Step: 5% Milk for 12 hours at 4°C.

    See Abreview


This product has been referenced in:
  • Vidyasagar A  et al. Tubular expression of heat-shock protein 27 inhibits fibrogenesis in obstructive nephropathy. Kidney Int 83:84-92 (2013). WB . Read more (PubMed: 22971995) »
  • Luksha N  et al. Impaired resistance artery function in patients with end-stage renal disease. Clin Sci (Lond) 120:525-36 (2011). Read more (PubMed: 21222655) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (HaCaT)
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (15%, 6V, 60 min.)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Mar 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Normal Skin)
Normal Skin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat, pH 6.0

Abcam user community

Verified customer

Submitted Jan 27 2010


Thank you for your enquiry. Hsp27 is cytoplasmic in interphase cells, colocalizes with mitotic spindles in mitotic cells and translocates to the nucleus during heat shock. I would recommend that your customer uses a RIPA buffer extraction. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry. I am sorry to hear that your customer has been having difficulties with this antibody. I have read your customer's questionaire and they are following a protocol that I would largely recommend. However, I would appreciate it if you could tell me how they are inducing hsp27 as I am unfamiliar with an induction by hsp70. Can you please ask your customer to clarify how this is performed and whether they can be certain of the expression of hsp27 in HT1080 cells. From the literature it is clear that the expression of this target is variable across cell types and cell lines. I would therefore be interested in further information. We recommend that a breast carcinoma cell line is used as a positive control. I look forward to hearing from your customer.

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We do not know for sure whether this anitbody recognises phosphorylated and non-phosphorylated forms of HSP27. It might be possible to find the answer in published work involving this antibody - there are five listed reference on the datasheet. We do not stock an antibody to HSP27 for which we can confidently claim that it will only recognize the phosphorylated form of HSP27.

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