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Full length protein corresponding to Human Hsp27. Partially purified HSP27 derived from MCF-7 cytosol.
Our Abpromise guarantee covers the use of ab8600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||1/50 - 1/100.|
|IHC-Fr||1/50 - 1/100.|
|IHC-P||1/50 - 1/100.|
|WB||1/100 - 1/300.|
ab8600 staining Hsp27 in human normal skin tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation and heat mediated antigen retrieval in Citrate buffer pH 6.0. The primary antibody was diluted, 1/50 in PBT (PBS + 0.5% Tween-20 + 0.5% BSA) and incubated with sample for 20 hours at 21°C. An Alexa Fluor® 594 conjugated Donkey polyclonal to mouse IgG
was used as secondary at 1/100 dilution, for 1 hour at 21°C. ab8600 show red staining of Hsp27 in image. Counterstain with DAPI was performed by adding DAPI in a final concentration of 0.5 µg/ml to the mounting medium.
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