Product nameAnti-Hsp27 antibody [B317]
See all Hsp27 primary antibodies
DescriptionMouse monoclonal [B317] to Hsp27
SpecificityThis antibody, also known as the estrogen regulated 24 kD protein and HSP 28, is one of the several heat shock proteins (HSP) produced by all organisms studied. The antigen is induced by elevated temperature as well as estrogen in hormone response. This protein has been studied in human estrogen response tissues such as cervix, endometrium and breast tissue. It may be useful in classification of various hormone sensitive tumors.
Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, IHC-P, WBmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human Hsp27. Partially purified HSP27 derived from MCF-7 cytosol.
- Breast Carcinoma.
General notesProlonged fixation in buffered formalin can destroy the epitope.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab8600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||1/50 - 1/100.|
|IHC-Fr||1/50 - 1/100.|
|IHC-P||1/50 - 1/100.|
|WB||1/100 - 1/300.|
FunctionInvolved in stress resistance and actin organization.
Tissue specificityDetected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Involvement in diseaseDefects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
modificationsPhosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
- Information by UniProt
- Heat shock 27kDa protein antibody
- 28 kDa heat shock protein antibody
- CMT2F antibody
All lanes : Anti-Hsp27 antibody [B317] (ab8600) at 1/300 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 23 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa (possible post-translational modification)
ab8600 staining Hsp27 in human normal skin tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation and heat mediated antigen retrieval in Citrate buffer pH 6.0. The primary antibody was diluted, 1/50 in PBT (PBS + 0.5% Tween-20 + 0.5% BSA) and incubated with sample for 20 hours at 21°C. An Alexa Fluor® 594 conjugated Donkey polyclonal to mouse IgG
was used as secondary at 1/100 dilution, for 1 hour at 21°C. ab8600 show red staining of Hsp27 in image. Counterstain with DAPI was performed by adding DAPI in a final concentration of 0.5 µg/ml to the mounting medium.
All lanes : Anti-Hsp27 antibody [B317] (ab8600) at 0.83 µg/ml
All lanes : Human HaCaT Whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : An HRP-conjugated goat anti-mouse polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 minutes
Blocking Step: 5% Milk for 12 hours at 4°C.
This product has been referenced in:
- Vidyasagar A et al. Tubular expression of heat-shock protein 27 inhibits fibrogenesis in obstructive nephropathy. Kidney Int 83:84-92 (2013). WB . Read more (PubMed: 22971995) »
- Luksha N et al. Impaired resistance artery function in patients with end-stage renal disease. Clin Sci (Lond) 120:525-36 (2011). Read more (PubMed: 21222655) »