Anti-Hsp27 antibody [G3.1] (ab2790)
- Datasheet
- References (29)
- Protocols
Overview
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Product nameAnti-Hsp27 antibody [G3.1]
See all Hsp27 primary antibodies -
DescriptionMouse monoclonal [G3.1] to Hsp27
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Host speciesMouse
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Tested applicationsSuitable for: Flow Cyt, IHC-Fr, ICC/IF, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Chicken, Human, Non human primates
Predicted to work with: Cow, Pig
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Immunogen
Full length native protein (purified) corresponding to Human Hsp27. (Partially purified human HSP27)
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Positive control
- Hela Cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
PurityAscites
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ClonalityMonoclonal
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Clone numberG3.1
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab2790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | 1/100. | |
| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use citrate buffer. |
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| WB | 1/1000. |
Target
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FunctionInvolved in stress resistance and actin organization.
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Tissue specificityDetected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
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Involvement in diseaseDefects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs. -
Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
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Post-translational
modificationsPhosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock. -
Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
- Information by UniProt
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Database links
- Entrez Gene: 396227 Chicken
- Entrez Gene: 516099 Cow
- Entrez Gene: 3315 Human
- Entrez Gene: 15507 Mouse
- Entrez Gene: 493184 Pig
- Entrez Gene: 24471 Rat
- Entrez Gene: 101115754 Sheep
- Omim: 602195 Human
see all -
Alternative names
- Heat shock 27kDa protein antibody
- 28 kDa heat shock protein antibody
- CMT2F antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [G3.1] (ab2790)](https://www.abcam.com/ps/products/2/ab2790/Images/ab2790_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [G3.1] (ab2790)This image is courtesy of Michael Mancini, Ph.D.Cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/200 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody, goat anti-rabbit Alexa 594 (ab150080), was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minutes in
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [G3.1] (ab2790)](https://www.abcam.com/ps/products/2/ab2790/Images/ab2790-279726-ab2790-IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [G3.1] (ab2790)Immunohistochemistry of paraffin embedded human breast carcinoma with ab2790 labeling Hsp27 at 0.5μg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [G3.1] (ab2790)](https://www.abcam.com/ps/products/2/ab2790/Images/ab2790-279724-Ab2790-IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [G3.1] (ab2790)Immunohistochemistry of paraffin embedded human prostate carcinoma with ab2790 labeling Hsp27 at 0.5μg/ml.
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![Western blot - Anti-Hsp27 antibody [G3.1] (ab2790)](https://www.abcam.com/ps/products/2/ab2790/Images/Hsp27-Primary-antibodies-ab2790-2.jpg)
Western blot - Anti-Hsp27 antibody [G3.1] (ab2790)This image is courtesy of an Abreview submitted by Brian HittAnti-Hsp27 antibody [G3.1] (ab2790) at 1/1000 dilution + Mouse whole brain tissue lysate. at 10 µg
Secondary
An HRP-conjugated Goat polyclonal. at 1/10000 dilution
Developed using the ECL technique.
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Blocking Step: 5% Milk for 1 hour at 25°C.
Gel Running Conditions: Reduced, Denaturing Bis-tris 4-12% -
![Flow Cytometry - Anti-Hsp27 antibody [G3.1] (ab2790)](https://www.abcam.com/ps/products/2/ab2790/Images/Hsp27-Primary-antibodies-ab2790-10.jpg)
Flow Cytometry - Anti-Hsp27 antibody [G3.1] (ab2790)Overlay histogram showing HeLa cells (ab150035) stained with ab2790 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2790, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
References
This product has been referenced in:
- Sun P et al. Foot-and-mouth disease virus capsid protein VP2 activates the cellular EIF2S1-ATF4 pathway and induces autophagy via HSPB1. Autophagy 14:336-346 (2018). Read more (PubMed: 29166823) »
- San Gil R et al. Using bicistronic constructs to evaluate the chaperone activities of heat shock proteins in cells. Sci Rep 7:2387 (2017). Read more (PubMed: 28539657) »
