Anti-Hsp27 (phospho S82) antibody (ab90537)

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Western blot - Anti-Hsp27 (phospho S82) antibody (ab90537)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S82) antibody (ab90537)

Key features and details

  • Rabbit polyclonal to Hsp27 (phospho S82)
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Hsp27 (phospho S82) antibody
    See all Hsp27 primary antibodies

  • Description

    Rabbit polyclonal to Hsp27 (phospho S82)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Synthetic phosphopeptide from a region around serine 82 (Human) conjugated to KLH

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab90537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
Application Abreviews Notes
WB
1/2000. Predicted molecular weight: 23 kDa.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
1/2000. Predicted molecular weight: 23 kDa.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Involved in stress resistance and actin organization.

  • Tissue specificity

    Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

  • Involvement in disease

    Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
    Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.

  • Sequence similarities

    Belongs to the small heat shock protein (HSP20) family.

  • Post-translational
    modifications

    Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt

  • Database links

    • Alternative names

      • Heat shock 27kDa protein antibody
      • 28 kDa heat shock protein antibody
      • CMT2F antibody
      • DKFZp586P1322 antibody
      • epididymis secretory protein Li 102 antibody
      • Estrogen regulated 24 kDa protein antibody
      • Estrogen-regulated 24 kDa protein antibody
      • Heat shock 25kDa protein 1 antibody
      • Heat shock 27 kDa protein antibody
      • Heat shock 27kD protein 1 antibody
      • Heat shock 27kDa protein 1 antibody
      • Heat shock 28kDa protein 1 antibody
      • Heat Shock Protein 27 antibody
      • Heat shock protein beta 1 antibody
      • Heat shock protein beta-1 antibody
      • heat shock protein family B (small) member 1 antibody
      • HEL-S-102 antibody
      • HMN2B antibody
      • HS.76067 antibody
      • Hsp 25 antibody
      • HSP 27 antibody
      • Hsp 28 antibody
      • Hsp B1 antibody
      • Hsp25 antibody
      • HSP27 antibody
      • Hsp28 antibody
      • HspB1 antibody
      • HSPB1_HUMAN antibody
      • SRP27 antibody
      • Stress responsive protein 27 antibody
      • Stress-responsive protein 27 antibody
      see all

    Images

    • Western blot - Anti-Hsp27 (phospho S82) antibody (ab90537)
      Western blot - Anti-Hsp27 (phospho S82) antibody (ab90537)
      All lanes : Anti-Hsp27 (phospho S82) antibody (ab90537) at 1/2000 dilution

      Lane 1 : phosphorylated Hsp27 protein
      Lane 2 : Non-phosphorylated Hsp27 protein

      Predicted band size: 23 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S82) antibody (ab90537)
      Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S82) antibody (ab90537)
      ab90537 staining Hsp27 in HeLa cells by Confocal Immunofluorescence (green). Nuclei are stained in blue pseudocolor using DRAQ5.

    References (3)

    Publishing research using ab90537? Please let us know so that we can cite the reference in this datasheet.

    ab90537 has been referenced in 3 publications.

    • Drexler R  et al. Significance of unphosphorylated and phosphorylated heat shock protein 27 as a prognostic biomarker in pancreatic ductal adenocarcinoma. J Cancer Res Clin Oncol N/A:N/A (2020). IHC-P ; Human . PubMed: 32200459
    • Jovcevski B  et al. Phosphomimics destabilize Hsp27 oligomeric assemblies and enhance chaperone activity. Chem Biol 22:186-95 (2015). PubMed: 25699602
    • Li KC  et al. Reduced expression of HSP27 following HAD-B treatment is associated with Her2 downregulation in NIH:OVCAR-3 human ovarian cancer cells. Mol Med Rep 12:3787-94 (2015). PubMed: 26044344

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Question

    - Antibody binding non specifically, too many bands
    - 5% BSA
    - Human podocytes lysates
    - Sample heated in LDS buffer 5 min 99C.
    - Experiment repeated twice
    - Secondary antibody is OK

    Read More
    Answer

    According to our records, ab90537 was proving difficult to use inWB and we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.

    If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.

    We wish you the best of luck with your research and look forward to a reply.

    Read More

    Question

    - Antibody binding non specifically, too many bands
    - 5% BSA
    - Human podocytes lysates
    - Sample heated in LDS buffer 5 min 99C.
    - Experiment repeated twice
    - Secondary antibody is OK

    Read More
    Answer

    Thank you for contacting us.

    Please send image as we discussed.

    I will be looking forward tohearing from you soon.

    Read More

    Question

    we tried diferrent concentration. Pleaserefund all of them.

    Thanks,

    Read More
    Answer

    Thank you for your reply.

    Your credit note IDsare *******.

    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Question

    Please fix the problem for us. Attached are the procedure of experiment

    Thanks,

    Read More
    Answer

    Thank you for contacting Abcam.

    I am sorry about the issues you have been having with a few of our antibodies. These antibodies are covered under our Abpromise for six months and are guaranteed to work in western blot on rat samples .

    For ab47455 and ab49919, I do not think there is any protocol advice I can give that would help resolve this issue, therefore I would be happy to either replace or refund the cost of them. Please let me know how you would like to proceed for these 2 and if you do wish to receive replacements, include the catalogue numbers of the products you would like to receive.

    As for ab90537, I see that you used a primary antibody concentration of 1/2000, did you try to use any higher dilutions, say 1/500, with overnight incubation at 4C, to see if that helps resolve the issue?

    Looking forward to your reply and hope your day is going well.

    Read More

    Question

    anti phospho Hsp27 recomended antibodies.

    Read More
    Answer

    Thank you for contacting us.

    As discussed any anti phopho HsP27 specific antibody can be used.

    Please find attached the link to publication where author have used podocyte and anti Hsp27 p83 ab.

    http://ajprenal.physiology.org/content/early/2008/10/15/ajprenal.90234.2008.full.pdf

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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