Anti-Hsp27 (phospho S82) antibody (ab90537)

Submit a review Q&A (5)References (2)

Overview

  • Product name

    Anti-Hsp27 (phospho S82) antibody
    See all Hsp27 primary antibodies

  • Description

    Rabbit polyclonal to Hsp27 (phospho S82)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Chicken, Guinea pig, Cow, Dog, Human

  • Immunogen

    Synthetic phosphopeptide from a region around serine 82 (Human) conjugated to KLH

  • Positive control

    • Heat shocked HeLa cells; phosphorylated Hsp27 protein. This antibody gave a positive result in IHC in the following FFPE tissue: Human skeletal muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab90537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 23 kDa.
IP 1/100.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in stress resistance and actin organization.

  • Tissue specificity

    Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

  • Involvement in disease

    Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
    Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.

  • Sequence similarities

    Belongs to the small heat shock protein (HSP20) family.

  • Post-translational
    modifications

    Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Heat shock 27kDa protein antibody
    • 28 kDa heat shock protein antibody
    • CMT2F antibody
    • DKFZp586P1322 antibody
    • epididymis secretory protein Li 102 antibody
    • Estrogen regulated 24 kDa protein antibody
    • Estrogen-regulated 24 kDa protein antibody
    • Heat shock 25kDa protein 1 antibody
    • Heat shock 27 kDa protein antibody
    • Heat shock 27kD protein 1 antibody
    • Heat shock 27kDa protein 1 antibody
    • Heat shock 28kDa protein 1 antibody
    • Heat Shock Protein 27 antibody
    • Heat shock protein beta 1 antibody
    • Heat shock protein beta-1 antibody
    • heat shock protein family B (small) member 1 antibody
    • HEL-S-102 antibody
    • HMN2B antibody
    • HS.76067 antibody
    • Hsp 25 antibody
    • HSP 27 antibody
    • Hsp 28 antibody
    • Hsp B1 antibody
    • Hsp25 antibody
    • HSP27 antibody
    • Hsp28 antibody
    • HspB1 antibody
    • HSPB1_HUMAN antibody
    • SRP27 antibody
    • Stress responsive protein 27 antibody
    • Stress-responsive protein 27 antibody
    see all

Images

  • Western blot - Anti-Hsp27 (phospho S82) antibody (ab90537)
    Western blot - Anti-Hsp27 (phospho S82) antibody (ab90537)
    All lanes : Anti-Hsp27 (phospho S82) antibody (ab90537) at 1/2000 dilution

    Lane 1 : phosphorylated Hsp27 protein
    Lane 2 : Non-phosphorylated Hsp27 protein

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S82) antibody (ab90537)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S82) antibody (ab90537)

    IHC image of Hsp27 (phospho S82) staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90537, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S82) antibody (ab90537)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S82) antibody (ab90537)
    ab90537 staining Hsp27 in HeLa cells by Confocal Immunofluorescence (green). Nuclei are stained in blue pseudocolor using DRAQ5.

References

This product has been referenced in:

  • Jovcevski B  et al. Phosphomimics destabilize Hsp27 oligomeric assemblies and enhance chaperone activity. Chem Biol 22:186-95 (2015). Read more (PubMed: 25699602) »
  • Li KC  et al. Reduced expression of HSP27 following HAD-B treatment is associated with Her2 downregulation in NIH:OVCAR-3 human ovarian cancer cells. Mol Med Rep 12:3787-94 (2015). Read more (PubMed: 26044344) »
See all 2 Publications for this product

Publishing research using ab90537? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Question

- Antibody binding non specifically, too many bands
- 5% BSA
- Human podocytes lysates
- Sample heated in LDS buffer 5 min 99C.
- Experiment repeated twice
- Secondary antibody is OK

Read More
Answer

According to our records, ab90537 was proving difficult to use inWB and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.

If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.

We wish you the best of luck with your research and look forward to a reply.

Read More

Question

- Antibody binding non specifically, too many bands
- 5% BSA
- Human podocytes lysates
- Sample heated in LDS buffer 5 min 99C.
- Experiment repeated twice
- Secondary antibody is OK

Read More
Answer

Thank you for contacting us.

Please send image as we discussed.

I will be looking forward tohearing from you soon.

Read More

Question

we tried diferrent concentration. Pleaserefund all of them.

Thanks,

Read More
Answer

Thank you for your reply.

Your credit note IDsare *******.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Question

Please fix the problem for us. Attached are the procedure of experiment

Thanks,

Read More
Answer

Thank you for contacting Abcam.

I am sorry about the issues you have been having with a few of our antibodies. These antibodies are covered under our Abpromise for six months and are guaranteed to work in western blot on rat samples .

For ab47455 and ab49919, I do not think there is any protocol advice I can give that would help resolve this issue, therefore I would be happy to either replace or refund the cost of them. Please let me know how you would like to proceed for these 2 and if you do wish to receive replacements, include the catalogue numbers of the products you would like to receive.

As for ab90537, I see that you used a primary antibody concentration of 1/2000, did you try to use any higher dilutions, say 1/500, with overnight incubation at 4C, to see if that helps resolve the issue?

Looking forward to your reply and hope your day is going well.

Read More

Question

anti phospho Hsp27 recomended antibodies.

Read More
Answer

Thank you for contacting us.

As discussed any anti phopho HsP27 specific antibody can be used.

Please find attached the link to publication where author have used podocyte and anti Hsp27 p83 ab.

http://ajprenal.physiology.org/content/early/2008/10/15/ajprenal.90234.2008.full.pdf

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com