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Synthetic phosphopeptide derived from a region of mouse HSP25 that contains serine 86.
Our Abpromise guarantee covers the use of ab17938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
|IHC-Fr||Use at an assay dependent concentration. See Abreview.|
|ICC/IF||Use at an assay dependent concentration. See Abreview.|
Hsp25 (phospho S86) antibody image 6534.
Western blot using ab17938 on NIH3T3 cells treated with anisomycin.
Lane 1: unstimulated cells
Lane 2: cells stimulated with anisomycin
Lane 3: cells stimulated with anisomycin. Antibody blocked with the non-phosphopeptide corresponding to the immunogen
Lane 4: cells stimulated with anisomycin. Antibody blocked with generic phosphoserine-containing peptide
Lane 5: cells stimulated with anisomycin. Antibody blocked with the phosphopeptide immunogen
Lane 6: cells stimulated with anisomycin and treated with lambda phosphatase
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