Product nameAnti-HSP31 antibody
DescriptionGoat polyclonal to HSP31
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Saccharomyces cerevisiae
- Lysates of postdiauxic, overnight grown yeast cells.
Previously labelled as Hsp31p.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab156936 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab156936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.02 - 0.06 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa).|
RelevanceProbable protease. May act as a chaperone.
- D9719.36 antibody
- Heat shock protein 31 antibody
- Hsp31p antibody
All lanes : Anti-HSP31 antibody (ab156936) at 0.05 µg/ml
Lane 1 : Wild type yeast lysate
Lane 2 : KO yeast lysate
Lane 3 : Wild type yeast lysate, Ponceau stain
Lane 4 : KO yeast lysate, Ponceau stain
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 26 kDa
Approx 24 kDa band observed in lysates of postdiauxic (overnight grown yeast cells) and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected.
ab156936 has not yet been referenced specifically in any publications.