Overview

  • Product name
    Anti-HSP31 antibody
  • Description
    Goat polyclonal to HSP31
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to Saccharomyces cerevisiae HSP31 aa 50-64 (internal sequence) (Cysteine residue).
    Sequence:

    ETGKFGWDEHSLAKD


    Database link: NP_010822.1

  • Positive control
    • Lysates of postdiauxic, overnight grown yeast cells.
  • General notes

    Previously labelled as Hsp31p.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab156936 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.02 - 0.06 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa).

Target

  • Relevance
    Probable protease. May act as a chaperone.
  • Database links
    • Alternative names
      • D9719.36 antibody
      • Heat shock protein 31 antibody
      • Hsp31p antibody
      • Probable chaperone protein HSP31 antibody
      • YDR533C antibody
      see all

    Images

    • All lanes : Anti-HSP31 antibody (ab156936) at 0.05 µg/ml

      Lane 1 : Wild type yeast lysate
      Lane 2 : KO yeast lysate
      Lane 3 : Wild type yeast lysate, Ponceau stain
      Lane 4 : KO yeast lysate, Ponceau stain

      Lysates/proteins at 35 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 26 kDa



      Approx 24 kDa band observed in lysates of postdiauxic (overnight grown yeast cells) and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected.

    References

    ab156936 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab156936.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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