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Goat polyclonal to Hsp31p
by a Cysteine residue linker, corresponding to internal sequence amino acids 50-64 of Saccharomyces cerevisiae Hsp31p (NP_010822.1).
Lysates of postdiauxic, overnight grown yeast cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
Immunogen affinity purified
ab156936 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.02 - 0.06 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa).
Probable protease. May act as a chaperone.
Heat shock protein 31 antibody
Probable chaperone protein HSP31 antibody
Western blot - Anti-Hsp31p antibody (ab156936)
All lanes : Anti-Hsp31p antibody (ab156936) at 0.05 µg/ml Lane 1 : Wild type yeast lysate Lane 2 : KO yeast lysate Lane 3 : Wild type yeast lysate, Ponceau stain Lane 4 : KO yeast lysate, Ponceau stain Lysates/proteins at 35 µg per lane. Developed using the ECL technique. Predicted band size: 26 kDa Approx 24 kDa band observed in lysates of postdiauxic (overnight grown yeast cells) and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected.
has not yet been referenced specifically in any publications.
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