Anti-Hsp40 antibody [3B9.E6] (ab223607)
Key features and details
- Mouse monoclonal [3B9.E6] to Hsp40
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Hsp40 antibody [3B9.E6]
See all Hsp40 primary antibodies -
Description
Mouse monoclonal [3B9.E6] to Hsp40 -
Host species
Mouse -
Specificity
Does not cross-react with HDJ2 or YDJ1.
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Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Hsp40.
Database link: P25685 -
Positive control
- ICC/IF: HaCaT cells. WB: A431, A549, Daudi, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC-3 and T98G cell lysate; rat brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3B9.E6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Hep G2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A-431 whole cell lysate (ab30132)
- Jurkat whole cell lysate (ab7899)
- HEK-293 whole cell lysate (ab7902)
- A-431 whole cell lysate (ab7909)
- HL-60 whole cell lysate (ab7914)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/2000. Predicted molecular weight: 38 kDa.
|
ICC/IF |
1/100.
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Notes |
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WB
1/2000. Predicted molecular weight: 38 kDa. |
ICC/IF
1/100. |
Target
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Function
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. -
Sequence similarities
Contains 1 J domain. -
Cellular localization
Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. - Information by UniProt
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Database links
- Entrez Gene: 3337 Human
- Entrez Gene: 361384 Rat
- Omim: 604572 Human
- SwissProt: P25685 Human
- Unigene: 515210 Human
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Alternative names
- DnaJ (Hsp40) homolog subfmaily B member 1 antibody
- DNAJ 1 antibody
- DNAJ B1 antibody
see all
Images
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All lanes : Anti-Hsp40 antibody [3B9.E6] (ab223607) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DNAJB1 knockout HEK293T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab223607 observed at 39 kDa. Red - loading control ab52901 observed at kDa.
ab223607 Anti-Hsp40 antibody [3B9.E6] was shown to specifically react with Hsp40 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266344 (knockout cell lysate ab258400) was used. Wild-type and Hsp40 knockout samples were subjected to SDS-PAGE. ab223607 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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100% methanol fixed HaCat (human keratinocyte cell line) cells stained for Hsp40 (green) using ab223607 (1/100) in ICC/IF. Secondary Antibody: FITC Goat Anti-Mouse 1/50.
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All lanes : Anti-Hsp40 antibody [3B9.E6] (ab223607) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : HepG2 cell lysate
Lane 7 : HL-60 cell lysate
Lane 8 : HUVEC cell lysate
Lane 9 : Jurkat cell lysate
Lane 10 : MCF7 cell lysate
Lane 11 : PC-3 cell lysate
Lane 12 : T98G cell lysate
Lane 13 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Sheep Anti-Mouse IgG HRP
Predicted band size: 38 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab223607 has been referenced in 1 publication.
- Jeeva S et al. Hantavirus RdRp requires a host cell factor for cap snatching. J Virol N/A:N/A (2018). PubMed: 30541836