Overview

  • Product name

    Anti-Hsp40 antibody [3B9.E6]
    See all Hsp40 primary antibodies
  • Description

    Mouse monoclonal [3B9.E6] to Hsp40
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Hsp40.
    Database link: P25685

  • Positive control

    • ICC/IF: HaCaT cells. WB: A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC-3 and T98G cell lysate; rat brain lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    3B9.E6
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 38 kDa.
ICC/IF 1/100.

Target

  • Function

    Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
  • Sequence similarities

    Contains 1 J domain.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
  • Information by UniProt
  • Database links

  • Alternative names

    • DnaJ (Hsp40) homolog subfmaily B member 1 antibody
    • DNAJ 1 antibody
    • DNAJ B1 antibody
    • DnaJ heat shock protein family (Hsp40) member B1 antibody
    • DnaJ homolog subfamily B member 1 antibody
    • DnaJ protein homolog 1 antibody
    • DNAJ1 antibody
    • DNAJB 1 antibody
    • Dnajb1 antibody
    • DNAJB1 protein antibody
    • DNJB1_HUMAN antibody
    • HDJ 1 antibody
    • HDJ-1 antibody
    • HDJ1 antibody
    • Heat shock 40 kDa protein 1 antibody
    • Heat shock 40kD protein 1 antibody
    • Heat shock protein 40 antibody
    • Hsp 40 antibody
    • HSP40 antibody
    • HSPF 1 antibody
    • HSPF1 antibody
    • Human DnaJ protein 1 antibody
    • Radial spoke 16 homolog B antibody
    • RSPH16B antibody
    • Sis1 antibody
    see all

Images

  • 100% methanol fixed HaCat (human keratinocyte cell line) cells stained for Hsp40 (green) using ab223607 (1/100) in ICC/IF. Secondary Antibody: FITC Goat Anti-Mouse 1/50.

  • All lanes : Anti-Hsp40 antibody [3B9.E6] (ab223607) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HCT116 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : HEK293 cell lysate
    Lane 6 : HepG2 cell lysate
    Lane 7 : HL-60 cell lysate
    Lane 8 : HUVEC cell lysate
    Lane 9 : Jurkat cell lysate
    Lane 10 : MCF7 cell lysate
    Lane 11 : PC-3 cell lysate
    Lane 12 : T98G cell lysate
    Lane 13 : Rat brain lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Sheep Anti-Mouse IgG HRP

    Predicted band size: 38 kDa

References

This product has been referenced in:

See 1 Publication for this product

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