Key features and details
- Mouse monoclonal [3B9.E6] to Hsp40
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
Product nameAnti-Hsp40 antibody [3B9.E6]
See all Hsp40 primary antibodies
DescriptionMouse monoclonal [3B9.E6] to Hsp40
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Human Hsp40.
Database link: P25685
- ICC/IF: HaCaT cells. WB: A431, A549, Daudi, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC-3 and T98G cell lysate; rat brain lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
KO cell lines
KO cell lysates
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- Jurkat whole cell lysate (ab7899)
- HEK293 whole cell lysate (ab7902)
- A431 whole cell lysate (ab7909)
- A549 whole cell lysate (ab7910)
- HL60 whole cell lysate (ab7914)
Our Abpromise guarantee covers the use of ab223607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 38 kDa.|
FunctionInteracts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
Sequence similaritiesContains 1 J domain.
Cellular localizationCytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
- Information by UniProt
- DnaJ (Hsp40) homolog subfmaily B member 1 antibody
- DNAJ 1 antibody
- DNAJ B1 antibody
All lanes : Anti-Hsp40 antibody [3B9.E6] (ab223607) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DNAJB1 knockout HEK293T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Lanes 1-4: Merged signal (red and green). Green - ab223607 observed at 39 kDa. Red - loading control ab52901 observed at kDa.
ab223607 Anti-Hsp40 antibody [3B9.E6] was shown to specifically react with Hsp40 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266344 (knockout cell lysate ab258400) was used. Wild-type and Hsp40 knockout samples were subjected to SDS-PAGE. ab223607 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
100% methanol fixed HaCat (human keratinocyte cell line) cells stained for Hsp40 (green) using ab223607 (1/100) in ICC/IF. Secondary Antibody: FITC Goat Anti-Mouse 1/50.
All lanes : Anti-Hsp40 antibody [3B9.E6] (ab223607) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : HepG2 cell lysate
Lane 7 : HL-60 cell lysate
Lane 8 : HUVEC cell lysate
Lane 9 : Jurkat cell lysate
Lane 10 : MCF7 cell lysate
Lane 11 : PC-3 cell lysate
Lane 12 : T98G cell lysate
Lane 13 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
All lanes : Sheep Anti-Mouse IgG HRP
Predicted band size: 38 kDa
ab223607 has been referenced in 1 publication.
- Jeeva S et al. Hantavirus RdRp requires a host cell factor for cap snatching. J Virol N/A:N/A (2018). PubMed: 30541836